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Role of Interferon Regulatory Factor 5 (IRF-5) in CD4+ T cells during infection with L. donovani

Fabié, Aymeric; Hammami, Akil; Stäger, Simona . Role of Interferon Regulatory Factor 5 (IRF-5) in CD4+ T cells during infection with L. donovani In: 16e symposium annuel de parasitologie moléculaire du Québec, 6-7 juin 2016, Université McGill, Montréal, QC.

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Résumé

The Interferon Regulatory Factors (IRF) family plays a crucial role in many pathways. Nevertheless, the role of IRF-5 has been mainly studied in antigen presenting cells (dendritics cells, macrophages and B cells). This transcription factor is known to be implicated in antiviral responses, stimulating INF-I production, but it’s also involved in promoting pro-inflammatory cytokines such as TNF, IL-6 and IL-12. Recently, we showed that Irf5-/- mice have a defective Th1 response during visceral leishmaniasis. Furthermore, we also demonstrated that T cells could express IRF-5, at least at mRNA level, during the chronic phase of L. donovani infection. IRF-5 expression appeared to be TLR7 dependent, since it is absent in T cells from infected Tlr7-/- mice. In this study, we analyze the role of IRF-5 in CD4+ T cells during L. donovani infection. Surprisingly, IRF-5 was only upregulated in CD4+ T cells and in the spleen during chronic infection. IRF-5 translocation to the nucleus was assessed by ImageStream. To define the in vivo role of IRF-5 in CD4 T cells, we generated T cell-specific Irf5-/- mice. Interestingly, these mice demonstrated similar Leishmania-specific Th1, TFh and Tr1 responses to infected WT mice. In contrast, we observed a dramatic increase in the frequency of GrzB CD4+ T cells during chronic infection in the spleen and the liver. This correlated with a small decrease in the splenic parasite burden. Collectively, these results demonstrate for the first time the existence of IRF-5 in CD4+ T cells and provide new insights into its potential role in these cells.

Type de document: Document issu d'une conférence ou d'un atelier
Informations complémentaires: Affiche scientifique
Mots-clés libres: -
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 01 mai 2018 19:42
Dernière modification: 01 mai 2018 20:02
URI: http://espace.inrs.ca/id/eprint/5925

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