Fabié, Aymeric; Hammami, Akil et Stäger, Simona . Role of Interferon Regulatory Factor 5 (IRF-5) in CD4+ T cells during infection with L. donovani In: 16e symposium annuel de parasitologie moléculaire du Québec, 6-7 juin 2016, Université McGill, Montréal, QC.
Ce document n'est pas hébergé sur EspaceINRS.Résumé
The Interferon Regulatory Factors (IRF) family plays a crucial role in many pathways. Nevertheless, the role of IRF-5 has been mainly studied in antigen presenting cells (dendritics cells, macrophages and B cells). This transcription factor is known to be implicated in antiviral responses, stimulating INF-I production, but it’s also involved in promoting pro-inflammatory cytokines such as TNF, IL-6 and IL-12. Recently, we showed that Irf5-/- mice have a defective Th1 response during visceral leishmaniasis. Furthermore, we also demonstrated that T cells could express IRF-5, at least at mRNA level, during the chronic phase of L. donovani infection. IRF-5 expression appeared to be TLR7 dependent, since it is absent in T cells from infected Tlr7-/- mice. In this study, we analyze the role of IRF-5 in CD4+ T cells during L. donovani infection. Surprisingly, IRF-5 was only upregulated in CD4+ T cells and in the spleen during chronic infection. IRF-5 translocation to the nucleus was assessed by ImageStream. To define the in vivo role of IRF-5 in CD4 T cells, we generated T cell-specific Irf5-/- mice. Interestingly, these mice demonstrated similar Leishmania-specific Th1, TFh and Tr1 responses to infected WT mice. In contrast, we observed a dramatic increase in the frequency of GrzB CD4+ T cells during chronic infection in the spleen and the liver. This correlated with a small decrease in the splenic parasite burden. Collectively, these results demonstrate for the first time the existence of IRF-5 in CD4+ T cells and provide new insights into its potential role in these cells.
| Type de document: | Document issu d'une conférence ou d'un atelier |
|---|---|
| Informations complémentaires: | Affiche scientifique |
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 01 mai 2018 19:42 |
| Dernière modification: | 19 oct. 2023 13:15 |
| URI: | https://espace.inrs.ca/id/eprint/5925 |
Gestion Actions (Identification requise)
 |
Modifier la notice |