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Novel biological and chemical methods of chitin extraction from crustacean waste using saline water.

Pachapur, Vinayak Laxman; Guemiza, Karima; Rouissi, Tarek; Sarma, Saurabh Jyoti; Brar, Satinder Kaur (2016). Novel biological and chemical methods of chitin extraction from crustacean waste using saline water. Journal of Chemical Technology & Biotechnology , vol. 91 , nº 8. p. 2331-2339. DOI: 10.1002/jctb.4821.

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Résumé

Background Several techniques have been conventionally proposed to recover chitin from shrimp waste using large amount of freshwater and chemicals. The standard chemical recovery of chitin was first compared by replacing fresh water with seawater. In addition, biotechnological process with Bacillus subtilis (B1) and Bacillus licheniformis (B2) using seawater during all steps of chitin extraction was also studied. Results The demineralization rate (DM) was statistically significant when using seawater (p=0.000020) by chemical recovery in comparison to deproteinization (DP). Increasing HCl concentration (from 1 to 1.28 M) and reaction time (from 60 to 90 min) resulted in DM similar to fresh water (p>0.05). Highest DP rates were obtained with crude protease (B1; DP ≈74%) and (B2: DP ≈84%), when fermentation was carried out for 24 h at an enzyme ⁄substrate ratio of 2. Maximum DP was reached (≈79% for B1 and ≈82% for B2) after 15 days, while DM ranged between 55-60%. Conclusion Combined enzymatic DP (with B2) followed by chemical DM process was used to produce chitin (DP≈84%, DM≈94%) which on transformation to chitosan showed a degree of deacetylation equivalent to ≈71%. Combined approach could transform crustacean waste using sea water into chitin product of commercial value.

Type de document:
Informations complémentaires: Publication préliminaire en ligne: 23 Septembre 2015 Publication en ligne : 16 Octobre 2015
Mots-clés libres: crustacean waste; chitin; demineralization; deproteination; fermentation; seawater
Centre: Centre Eau Terre Environnement
Date de dépôt: 20 déc. 2016 15:09
Dernière modification: 20 déc. 2016 15:09
URI: http://espace.inrs.ca/id/eprint/3898

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