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Full Virulence of Pseudomonas aeruginosa Requires OprF

Fito-Boncompte, Laurene; Chapalain, Annelise; Bouffartigues, Emeline; Chaker, Hichem; Lesouhaitier, Olivier; Gicquel, Gwendoline; Bazire, Alexis; Madi, Amar; Connil, Nathalie ; Veron, Wilfried; Taupin, Laure; Toussaint, Bertrand; Cornelis, Pierre; Wei, Qing; Shioya, Koki; Déziel, Éric; Feuilloley, Marc G. J.; Orange, Nicole; Dufour, Alain; Chevalier, Sylvie (2011). Full Virulence of Pseudomonas aeruginosa Requires OprF Infection and Immunity , vol. 79 , nº 3. p. 1176-1186. DOI: 10.1128/iai.00850-10.

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Résumé

OprF is a general outer membrane porin of Pseudomonas aeruginosa, a well-known human opportunistic pathogen associated with severe hospital-acquired sepsis and chronic lung infections of cystic fibrosis patients. A multiphenotypic approach, based on the comparative study of a wild-type strain of P. aeruginosa, its isogenic oprF mutant, and an oprF-complemented strain, showed that OprF is required for P. aeruginosa virulence. The absence of OprF results in impaired adhesion to animal cells, secretion of ExoT and ExoS toxins through the type III secretion system (T3SS), and production of the quorum-sensing-dependent virulence factors pyocyanin, elastase, lectin PA-1L, and exotoxin A. Accordingly, in the oprF mutant, production of the signal molecules N-(3-oxododecanoyl)-L-homoserine lactone and N-butanoyl-L-homoserine lactone was found to be reduced and delayed, respectively. Pseudomonas quinolone signal (PQS) production was decreased, while its precursor, 4-hydroxy-2-heptylquinoline (HHQ), accumulated in the cells. Taken together, these results show the involvement of OprF in P. aeruginosa virulence, at least partly through modulation of the quorum-sensing network. This is the first study showing a link between OprF, PQS synthesis, T3SS, and virulence factor production, providing novel insights into virulence expression

Type de document:
Mots-clés libres: outer-membrane protein, iii secretion system, quinolone signal, exoenzyme-s, caenorhabditis elegans, biofilm formation, ompa protein, expression, fluorescens, model
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 30 avr. 2014 14:58
Dernière modification: 30 avr. 2014 14:58
URI: http://espace.inrs.ca/id/eprint/2202

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