Dépôt numérique
RECHERCHER

Full Virulence of Pseudomonas aeruginosa Requires OprF

Téléchargements

Téléchargements par mois depuis la dernière année

Plus de statistiques...

Fito-Boncompte, Laurene; Chapalain, Annelise; Bouffartigues, Emeline; Chaker, Hichem; Lesouhaitier, Olivier; Gicquel, Gwendoline; Bazire, Alexis; Madi, Amar; Connil, Nathalie ; Veron, Wilfried; Taupin, Laure; Toussaint, Bertrand; Cornelis, Pierre; Wei, Qing; Shioya, Koki; Déziel, Éric; Feuilloley, Marc G. J.; Orange, Nicole; Dufour, Alain et Chevalier, Sylvie (2011). Full Virulence of Pseudomonas aeruginosa Requires OprF Infection and Immunity , vol. 79 , nº 3. pp. 1176-1186. DOI: 10.1128/iai.00850-10.

[thumbnail of fito.pdf]
Prévisualisation
PDF
Télécharger (1MB) | Prévisualisation

Résumé

OprF is a general outer membrane porin of Pseudomonas aeruginosa, a well-known human opportunistic pathogen associated with severe hospital-acquired sepsis and chronic lung infections of cystic fibrosis patients. A multiphenotypic approach, based on the comparative study of a wild-type strain of P. aeruginosa, its isogenic oprF mutant, and an oprF-complemented strain, showed that OprF is required for P. aeruginosa virulence. The absence of OprF results in impaired adhesion to animal cells, secretion of ExoT and ExoS toxins through the type III secretion system (T3SS), and production of the quorum-sensing-dependent virulence factors pyocyanin, elastase, lectin PA-1L, and exotoxin A. Accordingly, in the oprF mutant, production of the signal molecules N-(3-oxododecanoyl)-L-homoserine lactone and N-butanoyl-L-homoserine lactone was found to be reduced and delayed, respectively. Pseudomonas quinolone signal (PQS) production was decreased, while its precursor, 4-hydroxy-2-heptylquinoline (HHQ), accumulated in the cells. Taken together, these results show the involvement of OprF in P. aeruginosa virulence, at least partly through modulation of the quorum-sensing network. This is the first study showing a link between OprF, PQS synthesis, T3SS, and virulence factor production, providing novel insights into virulence expression

Type de document: Article
Mots-clés libres: outer-membrane protein, iii secretion system, quinolone signal, exoenzyme-s, caenorhabditis elegans, biofilm formation, ompa protein, expression, fluorescens, model
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 30 avr. 2014 14:58
Dernière modification: 30 avr. 2014 14:58
URI: https://espace.inrs.ca/id/eprint/2202

Gestion Actions (Identification requise)

Modifier la notice Modifier la notice