Proanthocyanidin Interferes with Intrinsic Antibiotic Resistance Mechanisms of Gram-Negative Bacteria

Maisuria, Vimal B; Okshevsky, Mira; Déziel, Éric ORCID: https://orcid.org/0000-0002-4609-0115 et Tufenkji, Nathalie (2019). Proanthocyanidin Interferes with Intrinsic Antibiotic Resistance Mechanisms of Gram-Negative Bacteria Advanced Science , vol. 6 , nº 15: 1802333. pp. 1-12. DOI: 10.1002/advs.201802333.

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Résumé

Antibiotic resistance is spreading at an alarming rate among pathogenic bacteria in both medicine and agriculture. Interfering with the intrinsic resistance mechanisms displayed by pathogenic bacteria has the potential to make antibiotics more effective and decrease the spread of acquired antibiotic resistance. Here, it is demonstrated that cranberry proanthocyanidin (cPAC) prevents the evolution of resistance to tetracycline in Escherichia coli and Pseudomonas aeruginosa, rescues antibiotic efficacy against antibiotic-exposed cells, and represses biofilm formation. It is shown that cPAC has a potentiating effect, both in vitro and in vivo, on a broad range of antibiotic classes against pathogenic E. coli, Proteus mirabilis, and P. aeruginosa. Evidence that cPAC acts by repressing two antibiotic resistance mechanisms, selective membrane permeability and multidrug efflux pumps, is presented. Failure of cPAC to potentiate antibiotics against efflux pump-defective mutants demonstrates that efflux interference is essential for potentiation. The use of cPAC to potentiate antibiotics and mitigate the development of resistance could improve treatment outcomes and help combat the growing threat of antibiotic resistance.

Type de document:	Article
Mots-clés libres:	anti‐biofilm; antimicrobial; efflux pump; multidrug resistance; potentiation; potentiation
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	27 janv. 2020 16:47
Dernière modification:	07 févr. 2022 16:00
URI:	https://espace.inrs.ca/id/eprint/8583

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