Henderson, Melanie; Van Hulst, Andraea; Simoneau, Gabrielle; Barnett, Tracie A; Drapeau, Vicky; Mathieu, Marie-Ève; Nicolau, Belinda; Varin, Thibaut et Marette, André . Correlations between Measures of Adiposity Across Childhood and Adolescence and the Intestinal Microbiota in 15-17 Year-Old Children with a Family History of Obesity: Preliminary Findings from the QUALITY Cohort In: 57th Annual Meeting of the European Society for Paediatric Endocrinology (ESPE), 27-29 September 2018, Athens, Greece.
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Background: While differences in gut microbiota between obese and lean subjects have been described, few studies have ex-amined how adiposity across childhood relates to intestinal micro-biota composition and diversity in late adolescence.
Objective: To explore the correlations between measures of adiposity from childhood and adolescence with intestinal micro-biota composition and diversity at 15-17 years.
Methods: Data stem from the QUALITY cohort, a cohort study of 630 children with a parental history of obesity. Adiposity was assessed at 8-10 yrs, 10-12 yrs and 15-17 yrs. Height, weight and waist circumference were measured using standardized protocols, and body mass index z-scores (zBMI) were calculated and partici-pants were classified into weight categories according to CDC ref-erence standards. Percent fat mass was assessed using DXA. 16S-rRNA based microbial profiling of stool samples obtained from 22 participants at 15-17 yrs were conducted to determine the com-position and diversity of microbiota. Alpha-diversity indices used to assess richness include observed OTUs and the Chao1 index, whereas the Shannon and Simpson indices measured richness and evenness. Pearson’s correlations assessed associations between di-versity indices and measures of adiposity.
Results: Of the 22 participants, 14 were normal weight, 6 were overweight and 2 were obese. zBMI across all ages was negatively correlated with the Shannon and Simpson indices. In particular, the correlation of zBMI at 15-17 yrs with the Simp-son index was -0.41 (P=0.057). Similar, albeit weaker, negative correlations were noted with measures of evenness and percent fat mass, however none reached statistical significance. Waist circumference was also negatively associated with the Shannon and Simpson indices, with the strongest correlations being with waist circumference at 10-12 yrs and 15-17 yrs and the Simp-son index (r=-0.42, P=0.067 and -0.40, P=0.065 respectively). In contrast, measures of adiposity across all ages were positively correlated with measures of richness. The strongest correlations were between zBMI at 15-17 yrs (r=0.50, P= 0.019) and waist circumference at 15-17 yrs (r=0.39, P=0.075) and the Chao-1 index. At the genus level, we found a significantly greater abun-dance of Roseburia in the overweight/obese group compared to normal weight youth.
Conclusions: These preliminary data in a small sample of chil-dren suggest that increased adiposity in early life is associated with differences in gut microbiota diversity indices in late adolescence. The greater abundance of Roseburia, a butyrate producing bacte-ria, in the gut microbiota of the overweight/obese group remains to be confirmed in a larger sample size.
| Type de document: | Document issu d'une conférence ou d'un atelier |
|---|---|
| Informations complémentaires: | Hormones Research in Paediatrics (2018), 90 (suppl. 1), 43-44 Free Communications FC6.1 |
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 07 août 2019 15:39 |
| Dernière modification: | 07 août 2019 15:39 |
| URI: | https://espace.inrs.ca/id/eprint/8123 |
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