Dulcey Jordan, Carlos Eduardo; Déziel, Éric et Doucet, Nicolas . Semi-rational evolution of the RhlA enzyme from Pseudomonas aeruginosa for the synthesis of industrially relevant rhamnolipids In: 2nd Protein Engineering Canada Conference (PEC), 17-19 Juin 2016, Ottawa, Canada.
Ce document n'est pas hébergé sur EspaceINRS.Résumé
Rhamnolipids are non-toxic and biodegradable surfactants mainly produced by Pseudomonas aeruginosa. They demonstrate an excellent potential as substitu tes for synthetic surfactants and are currently found in formulations of household cleaning produ cts. Numerous other applications for these biosurfactants are currently evaluated in cosmetics, detergents and in the bioremediation of soils. The bacterial strains that produce rhamnolipids gener ate a mixture of congeners with different lipophilic chain lengths. In Pseudomonas aeruginosa, the predominant chain length is 10 carbons. Since the physicochemical properties of rhamnolipids a re directly influenced by their molecular structure, modification or improvement of their surfa ctant properties can be acquired by controlling the length of the carbon chains. In Pseudomonas aer uginosa, the enzyme RhlA is responsible for diverting the 10-carbon hydroxy fatty acids to the β-oxidation pathway, linking them to form dimer precursors of rhamnolipids. We have shown that it is possible to control the length of the fatty acid chain by changing the recognition pattern of the RhlA substrate through semi- rational mutagenesis. The main objective of this project is the functional and structural characterization of RhlA, improving its catalytic efficiency an d changing its affinity for hydroxy fatty acids with different chain lengths to produce rhamnolipids with distinct surfactant properties. Preliminary mutagenesis results of RhlA will be presented.
| Type de document: | Document issu d'une conférence ou d'un atelier |
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| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 25 juin 2018 15:53 |
| Dernière modification: | 09 déc. 2020 14:31 |
| URI: | https://espace.inrs.ca/id/eprint/5785 |
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