Chemical composition and anti-herpes simplex virus type 1 (HSV-1) activity of extracts from Cornus canadensis

Lavoie, Serge; Côté, Isabelle; Pichette, Andre; Gauthier, Charles; Ouellet, Micheal; Nagau-Lavoie, Francine; Mshvildadze, Vakhtang et Legault, Jean (2017). Chemical composition and anti-herpes simplex virus type 1 (HSV-1) activity of extracts from Cornus canadensis BMC Complementary and Alternative Medicine , vol. 17 , nº 1. p. 123. DOI: 10.1186/s12906-017-1618-2.

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Résumé

BACKGROUND: Many plants of boreal forest of Quebec have been used by Native Americans to treat a variety of microbial infections. However, the antiviral activities of these plants have been seldom evaluated on cellular models to validate their in vitro efficiencies. In this study, Cornus canadensis L. (Cornaceae), a plant used in Native American traditional medicine to treat possible antiviral infections, has been selected for further examination. METHODS: The plant was extracted by decoction and infusion with water, water/ethanol 1:1 and ethanol to obtain extracts similar to those used by Native Americans. The effects of the extracts were tested on herpes simplex virus type-1 (HSV-1) using a plaque reduction assay. Moreover, bioassay-guided fractionation was achieved to isolate bioactive compounds. RESULTS: Water/ethanol 1:1 infusion of C. canadensis leaves were the most active extracts to inhibit virus absorption with EC50 of about 9 mug mL-1, whereas for direct mode, both extraction methods using water or water/ethanol 1:1 as solvent were relatively similar with EC50 ranging from 11 to 17 mug mL-1. The fractionation led to the identification of active fractions containing hydrolysable tannins. Tellimagrandin I was found the most active compound with an EC50 of 2.6 muM for the direct mode and 5.0 muM for the absorption mode. CONCLUSION: Altogether, the results presented in this work support the antiviral activity of Cornus canadensis used in Native American traditional medicine.

Type de document:	Article
Mots-clés libres:	Cornus canadensis; HSV-1; Native American; Tellimagrandin I; Traditional medicine; hydrolysable tannins
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	25 juin 2017 14:03
Dernière modification:	25 juin 2017 14:03
URI:	https://espace.inrs.ca/id/eprint/5395

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