Lipid production by Yarrowia lipolytica grown on biodiesel-derived crude glycerol: optimization of growth parameters and their effects on the fermentation efficiency.

Sara, Magdouli; Brar, Satinder Kaur et Blais, Jean-François (2016). Lipid production by Yarrowia lipolytica grown on biodiesel-derived crude glycerol: optimization of growth parameters and their effects on the fermentation efficiency. RSC Advances , vol. 6 , nº 93. pp. 90547-90558. DOI: 10.1039/C6RA16382C.

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Résumé

La transcription des symboles et des caractères spéciaux utilisés dans la version originale de ce résumé n’a pas été possible en raison de limitations techniques. La version correcte de ce résumé peut être lue en PDF. Yarrowia lipolytica, a well-known oleaginous strain for single cell oil (SCO) production was grown in nitrogen-limited flask cultures. The effect of increasing the initial crude glycerol and nitrogen concentration was studied along the fermentation process. Significant biomass and SCO production was reported with a high initial glycerol concentration of 89 g L−1 and 0.54 g NH4OH per L during 6 h. Optimized culture conditions were tested using a 5 L fermenter during two-stage cultivation with a dissolved oxygen shift from 60% to 30% of dissolved oxygen corresponding to 50–80 h−1. Lipid concentration of 13.6 ± 0.8 g L−1 and lipid content 52.7 ± 1.2% (w/w of dry biomass) was obtained which is higher compared with literature values for Yarrowia species grown on crude glycerol based media. The yeast lipids contained mainly oleic, palmitic, linoleic and stearic acids which could serve as perfect precursors for the synthesis of biodiesel.

Type de document:	Article
Informations complémentaires:	Résumé avec symboles
Mots-clés libres:	biodiesel-derived glycerol; Yarrowia lipolytica; single cell oil; citric acid
Centre:	Centre Eau Terre Environnement
Date de dépôt:	26 avr. 2017 14:47
Dernière modification:	08 juin 2023 19:18
URI:	https://espace.inrs.ca/id/eprint/4744

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