Nipah virus matrix protein influences fusogenicity and is essential for particle infectivity and stability

Dietzel, Erik, Kolesnikova, Larissa, Sawatsky, Bevan, Heiner, Anja, Weis, Micheal, Kobinger, Gary, Becker, Stephan, von Messling, Véronika et Maisner, Andrea (2016). Nipah virus matrix protein influences fusogenicity and is essential for particle infectivity and stability Journal of Virology , vol. 90 , nº 5. p. 2514-2522. DOI: 10.1128/JVI.02920-15.

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Résumé

Nipah virus (NiV) causes fatal encephalitic infections in humans. To characterize the role of the matrix (M) protein in the viral life cycle, we generated a reverse genetics system based on NiV strain Malaysia. Using an enhanced green fluorescent protein (eGFP)-expressingMprotein-deleted NiV, we observed a slightly increased cell-cell fusion, slow replication kinetics, and significantly reduced peak titers compared to the parental virus. While increased amounts of viral proteins were found in the supernatant of cells infected with M-deleted NiV, the infectivity-to-particle ratio was more than 100-fold reduced, and the particles were less thermostable and of more irregular morphology. Taken together, our data demonstrate that theMprotein is not absolutely required for the production of cell-free NiV but is necessary for proper assembly and release of stable infectious NiV particles. © 2016, American Society for Microbiology.

Type de document:	Article
Mots-clés libres:	-
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	24 août 2016 14:10
Dernière modification:	24 août 2016 14:10
URI:	https://espace.inrs.ca/id/eprint/4515

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