Nipah virus matrix protein influences fusogenicity and is essential for particle infectivity and stability

Dietzel, Erik; Kolesnikova, Larissa; Sawatsky, Bevan; Heiner, Anja; Weis, Micheal; Kobinger, Gary; Becker, Stephan; von Messling, Véronika et Maisner, Andrea (2016). Nipah virus matrix protein influences fusogenicity and is essential for particle infectivity and stability Journal of Virology , vol. 90 , nº 5. pp. 2514-2522. DOI: 10.1128/JVI.02920-15.

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Résumé

Nipah virus (NiV) causes fatal encephalitic infections in humans. To characterize the role of the matrix (M) protein in the viral life cycle, we generated a reverse genetics system based on NiV strain Malaysia. Using an enhanced green fluorescent protein (eGFP)-expressingMprotein-deleted NiV, we observed a slightly increased cell-cell fusion, slow replication kinetics, and significantly reduced peak titers compared to the parental virus. While increased amounts of viral proteins were found in the supernatant of cells infected with M-deleted NiV, the infectivity-to-particle ratio was more than 100-fold reduced, and the particles were less thermostable and of more irregular morphology. Taken together, our data demonstrate that theMprotein is not absolutely required for the production of cell-free NiV but is necessary for proper assembly and release of stable infectious NiV particles. © 2016, American Society for Microbiology.

Type de document:	Article
Mots-clés libres:	-
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	24 août 2016 14:10
Dernière modification:	24 août 2016 14:10
URI:	https://espace.inrs.ca/id/eprint/4515

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