Low cost semi-continuous bioprocess and online monitoring of hydrogen production from crude glycerol.

Sarma, Saurabh Jyoti; Maiti, Sampa; Brar, Satinder Kaur; Le Bihan, Yann; Buelna, Gerardo et Verma, Mausam P. (2015). Low cost semi-continuous bioprocess and online monitoring of hydrogen production from crude glycerol. RSC Advances , vol. 5 , nº 109. pp. 89809-89816. DOI: 10.1039/C5RA14470A.

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Résumé

High process costs arising from expensive media components, hydrogen partial pressure buildup in the head-space of the reactor, sharp decrease in medium pH due to accumulation of organic acids, substrate inhibition, as well as loss of biomass due to short hydraulic retention times are the major bottlenecks of fermentative hydrogen production. Therefore, the purpose of the present investigation was to develop a process to deal with all these problems at a time. The proposed approach demonstrates enhanced hydrogen production by a low cost process involving crude glycerol (CG), the waste from the biodiesel production process, as the only feedstock. This semi-continuous type of fermentation carried out in a 7.5 L bioreactor helped to eliminate substrate inhibition with no compromise in cumulative hydrogen production. By controlling product inhibition and the negative effect of by-product accumulation, as well as by preventing the loss of active biomass, a production of 5.18 L-H₂ per L-medium has been achieved. This amount is higher than the 2.02 to 2.68 L-H₂ per L-medium previously reported for CG bioconversion. Reduction of feed CG concentration from 120 g L⁻¹ to 60 g L⁻¹ was found to improve the glycerol utilization from 65% to 91%.

Type de document:	Article
Mots-clés libres:	hydrogen production; costs; glycerol; substrate
Centre:	Centre Eau Terre Environnement
Date de dépôt:	23 avr. 2018 19:52
Dernière modification:	27 nov. 2019 15:22
URI:	https://espace.inrs.ca/id/eprint/3931

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