Zhang, Xiaolei; Yan, Song; Tyagi, Rajeshwar Dayal; Drogui, Patrick et Surampalli, Rao Y. (2014). Ultrasonication assisted lipid extraction from oleaginous microorganisms. Bioresource Technology , vol. 158 . pp. 253-261. DOI: 10.1016/j.biortech.2014.01.132.
Ce document n'est pas hébergé sur EspaceINRS.Résumé
Various solvents, including water, hexane, methanol, and chloroform/methanol (1:1 v/v), were tested to identify the efficiency of lipid extraction from Trichosporon oleaginosus and an oleaginous fungal strain SKF-5 under ultrasonication (520. kHz 40. W and 50. Hz 2800. W) and compared with the conventional chloroform methanol (2:1 v/v) extraction method. The highest lipid recovery 10.2% and 9.3% with water, 43.2% and 33.2% with hexane, 75.7% and 65.1% with methanol, 100% and 100% w/w biomass with chloroform/methanol were obtained from T. oleaginosus and SKF-5 strain, respectively, at ultrasonication frequency 50. Hz and power input 2800. W. Ultrasonication chloroform/methanol extraction recovered total lipid in a short time (15. min) and low temperature (25. °C). Whereas the conventional chloroform methanol extraction to achieve total lipid recovery required 12. h at 60. °C. Ultrasonication chloroform/methanol extraction would be a promising method of lipid extraction from the microorganisms.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | biodiesel; lipid extraction; oleaginous microorganism; ultrasonication |
| Centre: | Centre Eau Terre Environnement |
| Date de dépôt: | 11 avr. 2018 18:45 |
| Dernière modification: | 11 avr. 2018 18:45 |
| URI: | https://espace.inrs.ca/id/eprint/3698 |
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