Interaction between silver nanoparticles of 20 nm (AgNP20 ) and human neutrophils: induction of apoptosis and inhibition of de novo protein synthesis by AgNP20 aggregates

Poirier, Michelle; Simard, Jean-Christophe; Antoine, Francis et Girard, Denis (2014). Interaction between silver nanoparticles of 20 nm (AgNP20 ) and human neutrophils: induction of apoptosis and inhibition of de novo protein synthesis by AgNP20 aggregates Journal of Applied Toxicology , vol. 34 , nº 4. pp. 404-12. DOI: 10.1002/jat.2956.

Prévisualisation

PDF (Ce document est disponible à partir de la page 38 du mémoire à l'adresse URL suivante: http://espace.inrs.ca/2751/1/Dpt%20final%20de%20Michelle%20Poirier%20-%202015-01-14.pdf) - Image de couverture Télécharger (204kB) | Prévisualisation

Résumé

Cytotoxic and proinflammatory properties of silver nanoparticles (AgNPs) have been reported in few studies but the direct interaction between AgNPs and neutrophils, which play a key role in inflammation, has never been documented. Here, we examined the role of AgNPs with a starting size of 20 nm (AgNP20 ) in human neutrophils. Using dynamic light scattering for the characterization of NPs suspended under identical conditions to those used for in vitro experiments, we found that, at 10 microg ml(-1) , 92% of AgNP20 possess a diameter of 17.1 nm but, at 100 microg ml(-1) , a tri-modal size distribution with large aggregates was observed (> 500 nm). Neutrophil cell size increased when treated with AgNP20 and transmission electronic microscopy experiments revealed that AgNP20 can rapidly interact with the cell membrane, penetrate neutrophils, localize in vacuole-like structures, and be randomly distributed in the cytosol after 24 h. Treatment with 100 microg ml(-1) AgNP20 for 24 h (but not 10 microg ml(-1) ) increased the neutrophil apoptotic rate and inhibited de novo protein synthesis. We conclude that AgNP20 induced apoptosis and can act as potent inhibitors of de novo protein synthesis at 100, but not 10 microg ml(-1) in human neutrophils. Copyright (c) 2013 John Wiley & Sons, Ltd.

Type de document:	Article
Mots-clés libres:	inflammation; neutrophils; apoptosis; protein synthesis; silver nanoparticles
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	04 avr. 2017 14:41
Dernière modification:	04 avr. 2017 14:41
URI:	https://espace.inrs.ca/id/eprint/3092

Gestion Actions (Identification requise)

Modifier la notice