Johnson, Bruno; Opimba, Marlyse et Bernier, Jacques 
ORCID: https://orcid.org/0000-0002-0594-5922
(2013).
Implications of the O-GlcNAc modification in the regulation of nuclear apoptosis in T cells
Biochimica et Biophysica Acta
, vol. 1840
, nº 1.
pp. 191-198.
DOI: 10.1016/j.bbagen.2013.09.011.
Résumé
BACKGROUND: O-linked beta-N-acetylglucosamine (O-GlcNAc) is a nutrient-/stress-sensitive post-translational modification that affects nucleocytoplasmic proteins. The enzyme O-N-acetylglucosamine transferase (OGT) catalyzes the addition of O-GlcNAc, whereas O-N-acetylglucosaminidase (OGA) removes it. O-GlcNAcylation plays a role in fundamental regulatory mechanisms through the modification of proteins involved in cell division, metabolism, transcription, cell signaling and apoptosis. The effects of O-GlcNAcylation on apoptosis appear to be cell-dependent, as elevated levels played a protective role in primary neonatal rat ventricular myocytes but had a cytotoxic effect in rat pancreatic beta-cells. The aim of the current study was to determine the implications of the O-GlcNAc modification on T cell apoptosis. METHODS: Human T lymphoblastic HPB-ALL cells were treated with the OGA inhibitor O-(2-acetamido-2-deoxy-d-glucopyranosylidene) amino-N-phenylcarbamate (PUGNAc), or with glucosamine (GlcN), to increase O-GlcNAcylation. Apoptosis was induced in the presence of tributyltin (TBT). DNA fragmentation was observed by cell cycle analysis and corresponded to the sub G0/G1 population. O-GlcNAcylated proteins were detected by immunoblot using a specific antibody (ctd110.6) and were precipitated using succinylated wheat germ agglutinin (sWGA). RESULTS: HPB-ALL cells treated with PUGNAc displayed a significant reduction in DNA fragmentation after TBT-induced apoptosis. DFF45, the protein that inhibits the endonuclease DFF40, was identified to be O-GlcNAc modified. O-GlcNAcylated DFF45 appeared to be more resistant to caspase cleavage during apoptosis. Our results suggest that a decrease in the O-GlcNAc modification on DFF45 occurs before its cleavage by caspase. GENERAL SIGNIFICANCE: Our results indicate that the O-GlcNAcylation of DFF45 may represent a mechanism to control the accidental activation of DFF.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Apoptosis; DFF; DFF45; O-(2-acetamidO-2-deoxy-D-glucopyranosylidene) amino-N-phenylcarbamate; O-linked β-N-acetylglucosamine; TBT |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 19 mai 2017 16:05 |
| Dernière modification: | 16 févr. 2022 21:08 |
| URI: | https://espace.inrs.ca/id/eprint/2922 |
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