Trudeau, Karine; Vu, Khanh Dang; Déziel, Éric; Shareck, François et Lacroix, Monique (2014). Effect of γ-irradiation on gene expression of heat shock proteins in the foodborne pathogen Escherichia coli O157:H7 International Journal of Radiation Biology , vol. 90 , nº 3. pp. 268-273. DOI: 10.3109/09553002.2014.859766.
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Purpose: The expression levels of seven genes (clpB, dnaK, groES, grpE, htpG, htpX and ibpB) encoding heat shock proteins (HSP) in Escherichia coli O157:H7 (E. coli) gamma irradiated was investigated. Timing impact of post-irradiated RNA extn. on the expression levels of these seven genes was also studied at a dose damaging the bacterial cells (0.4 kGy). Methods: Bacterial samples were γ-irradiated at 0.4 kGy and at a LD of 1.3 kGy. RNA was extd. at 0 min post irradn. for both irradn. doses and at 15, 30, 60, 90 or 120 min post-irradn. at the dose damaging the cells. Quantification of the gene expression was performed using quant. real-time polymerase chain reaction (q-RT-PCR). Results: The expression of genes encoding HSP was a very dynamic process evolving rapidly when E. coli cells were irradiated at 0.4 kGy. Notably, groES, grpE and ibpB were more up- regulated at 1.3 kGy than those at 0.4 kGy. Conclusions: For the seven genes studied there were more damaged proteins during irradn. at the LD and this dose causes increased expression in HSP which contributes to damage reparation. Expression patterns of genes encoding HSP in E. coli treated by γ-irradn. are different from those treated by heat shock.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Gene expression, heat shock proteins, E. coli, irradiation, qRT-PCR |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 29 avr. 2014 18:54 |
| Dernière modification: | 31 mai 2018 20:22 |
| URI: | https://espace.inrs.ca/id/eprint/2180 |
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