Short-Chain Fatty Acid Utilization in Cyberlindnera jadinii for Single-Cell Protein and Odd-Chain Fatty Acid Production

Hermansen, Christian ORCID: https://orcid.org/0000-0002-2740-780X; Siao, Rowanne ORCID: https://orcid.org/0009-0000-5884-7501; Chua, Gi Gi; Lee, Mikko Ru Xuan; Thong, Aaron ORCID: https://orcid.org/0000-0003-1852-4067; Weingarten, Melanie ORCID: https://orcid.org/0000-0002-9946-0183; Lindley, Nic ORCID: https://orcid.org/0000-0002-9538-1306 et Peterson, Eric Charles ORCID: https://orcid.org/0000-0003-1513-6106 (2025). Short-Chain Fatty Acid Utilization in Cyberlindnera jadinii for Single-Cell Protein and Odd-Chain Fatty Acid Production Microorganisms , vol. 13 , nº 7. p. 1558. DOI: 10.3390/microorganisms13071558.

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Résumé

In view of the growing global need for sustainable protein sources, this study explores the utilization of short-chain fatty acids into single-cell protein using the non-conventional yeast Cyberlindnera jadinii. Short-chain fatty acids can be sustainably produced via anaerobic digestion of organic waste, presenting a promising fermentation substrate for a circular bioeconomy. Cyberlindnera jadinii is demonstrated to be capable of growing on acetate, propionate and butyrate as both a carbon and energy source without strong inhibition. Bioprocess development was conducted in stirred tank bioreactors, where a fed-batch pH-stat bioprocess led to improved efficiency without substrate inhibition. The highest titer of 31.3 ± 1.0 g/L, rate of 0.67 ± 0.02 g/L/h and yield of 0.36 ± 0.01 g/g was achieved with propionate. The resulting biomass contained 41.3% crude protein, and 17.3% crude lipids with 81% unsaturated fatty acids. In contrast to acetate and butyrate, propionate as a substrate led to accumulation of 37% odd-chain fatty acids with titer, rate and yield of 1.74 ± 0.06 g/L, 0.037 ± 0.001 g/L/h and 0.020 ± 0.001 g/g. These findings confirm that short-chain fatty acids are viable fermentation substrates not only for single-cell protein, but also unsaturated and odd-chain fatty acid production with Cyberlindnera jadinii.

Type de document:	Article
Mots-clés libres:	short-chain fatty acid; non-conventional yeast; Cyberlindnera jadinii; bioprocess development; single-cell protein; single-cell oil; odd-chain fatty acids; food; feed
Centre:	Centre Eau Terre Environnement
Date de dépôt:	08 sept. 2025 19:16
Dernière modification:	08 sept. 2025 19:16
URI:	https://espace.inrs.ca/id/eprint/16596

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