Quantification of Neural Progenitor Cells From Zika Virus-Infected Zebrafish Embryos

Gomes, Yago C P; Sow, Aïssatou Aïcha; Patten, Shunmoogum A. ORCID: https://orcid.org/0000-0002-2782-3547 et Chatel-Chaix, Laurent ORCID: https://orcid.org/0000-0002-7390-8250 (2025). Quantification of Neural Progenitor Cells From Zika Virus-Infected Zebrafish Embryos Bio-protocol , vol. 15 , nº 13:e5377. pp. 1-11. DOI: 10.21769/BioProtoc.5377.

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Résumé

Zika virus (ZIKV), an arthropod-borne orthoflavivirus, has emerged as a global health concern due to its ability to cause severe fetal neurological disorders, leading to the congenital Zika syndrome (CZS) in neonates. Vertical transmission during pregnancy can alter neural progenitor cell (NPC) proliferation and differentiation and induce apoptosis, leading to microcephaly and other neurodevelopmental abnormalities. While mammalian models have been used to study the impact of ZIKV on NPC behavior, limitations such as high costs, dedicated time, and ethical constraints have fostered the exploration of alternative systems. The zebrafish embryo constitutes an advantageous in vivo model for studying ZIKV neuropathogenesis. Indeed, ZIKV infection phenocopies several features of the CZS while sharing a conserved neuroanatomical layout and offering genetic plasticity and unique accessibility to the infected brain compared to mammals. Here, we describe a protocol for characterizing ZIKV-induced defects of NPCs in this zebrafish model, relying on whole animal flow cytometry.

Type de document:	Article
Informations complémentaires:	This protocol is used in: PLOS Pathog (2024), DOI: 10.1371/journal.ppat.1012756 Our work on zebrafish and ZIKV was supported by the Canada Foundation for Innovation (CFI; #37512, to S.P.; https://www.innovation.ca/), by the Canadian Institutes for Health Research (CIHR; PJT 190064 to L.C.C. and S.P., and PJT 177940 to S.P.; https://cihr-irsc.gc.ca/), by an Acceleration grant from the Centre d'Excellence en Recherche sur les Maladies Orphelines – Fondation Courtois (CERMO-FC; https://www.cermofc.uqam.ca/) to S.P. and L.C.C., and by a Azrieli Future Leader in Canadian Brain Research grant from Brain Canada Foundation (https://braincanada.ca/fr/) through the Canada Brain Research Fund, with the financial support of Health Canada and the Azrieli Foundation awarded to L.C.C. A.A.S. has received PhD fellowships from the Fonds de Recherche du Québec-Nature et Technologies (FRQNT; https://frq.gouv.qc.ca/nature-et-technologies/), the Armand-Frappier Foundation (https://fondationafrappier.ca/), and the CERMO-FC. L.C.C. is receiving a research scholar (Senior) salary support from Fonds de Recherche du Québec-Santé (FRQS; https://frq.gouv.qc.ca/sante/). S.P. holds an FRQS Junior 2 research scholar salary award and the Anna Sforza Djoukhadjian Research Chair. This protocol was used in [11].
Mots-clés libres:	Zebrafish; Zika virus; Neural progenitor cells; Flow cytometry
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	28 août 2025 18:56
Dernière modification:	28 août 2025 18:56
URI:	https://espace.inrs.ca/id/eprint/16581

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