Holocellulose nanofibrils as effective nisin immobilization substrates for antimicrobial food packaging

Huang, Kehao; Zhang, Cunzhi; Hu, Yuxin; Lacroix, Monique ORCID: https://orcid.org/0000-0002-2042-4033 et Wang, Yixiang (2025). Holocellulose nanofibrils as effective nisin immobilization substrates for antimicrobial food packaging International Journal of Biological Macromolecules , vol. 304 , nº Pt 1. pp. 1-9. DOI: 10.1016/j.ijbiomac.2025.140741.

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Résumé

Holocellulose nanofibrils (HCNF), a type of nanocellulose with abundant amorphous regions suitable for chemical modification, show promise for sustainable food packaging but remain underutilized. This study employed HCNF to immobilize nisin and then spray-coated on the surface of soy protein isolate (SPI) films to improve mechanical, barrier, and antimicrobial properties. HCNF was extracted from wood sheet through chemical delignification and low-energy defibrillation, and then oxidized to introduce aldehyde groups for efficient nisin conjugation. The abundant amorphous regions led to a high immobilization rate of 3.4 mg/g, and conjugated HCNF and nisin coatings on SPI films significantly enhanced tensile strength to 3.43 ± 0.09 MPa, reduced water vapor permeability to 2.48 ± 0.07 × 10(-6) g m(-1) h(-1) Pa(-1), and decreased oxygen permeability to 4.29 ± 0.46 × 10(-4) cm(3) m(-1) day(-1) atm(-1). The conjugate sustained inhibition of S. aureus and L. monocytogenes, and the coating of 9 wt% conjugate on SPI films resulted in a 6-log reduction in bacterial count for both bacteria, while free nisin lost its antimicrobial efficacy during 24 h of pre-incubation. This work suggests feasibility of using HCNF as an effective substrate for nisin immobilization, providing a sustainable functional packaging solution with extended antimicrobial activity.

Type de document:	Article
Informations complémentaires:	document: 140741
Mots-clés libres:	Holocellulose nanofibrils;Nisin immobilization; Antimicrobial food packaging
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	16 févr. 2025 04:14
Dernière modification:	16 févr. 2025 04:15
URI:	https://espace.inrs.ca/id/eprint/16317

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