Immunomodulatory properties of Leishmania tarentolae extracellular vesicles containing the Spike protein of SARS-CoV-2

Medina Jaramillo, Ana Catalina; Acevedo Ospina, Hamlet Adolfo et Descoteaux, Albert ORCID: https://orcid.org/0000-0002-0633-5309 (2024). Immunomodulatory properties of Leishmania tarentolae extracellular vesicles containing the Spike protein of SARS-CoV-2 Frontiiers in Parasitology , vol. 3 , nº 1306478. pp. 1-12. DOI: 10.3389/fpara.2024.1306478.

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Résumé

Extracellular vesicles released by the protozoan parasite Leishmania display immunomodulatory properties towards mammalian immune cells. In this study, we have evaluated the potential of extracellular vesicles derived from the non-pathogenic protozoan Leishmania tarentolae towards the development of a vaccine adjuvant. As a proof of concept, we expressed in L. tarentolae a codon-optimized SARS-CoV-2 Spike protein fused to the L. mexicana secreted acid phosphatase signal peptide in the N-terminal and to a 6×-His stretch in the C-terminal. Extracellular vesicles released by the engineered L. tarentolae were isolated by ultracentrifugation and fast protein liquid chromatography and were characterized via nanoparticle tracking analysis and transmission electron microscopy. The recombinant S protein was present in extracellular vesicles released by L. tarentolae, as determined by Western blot analyses and immunoelectron microscopy. Next, we evaluated the immunomodulatory potential of extracellular vesicles containing the S protein towards bone-marrow-derived macrophages and bone-marrow-derived dendritic cells. Our data show that in bone-marrow-derived dendritic cells, extracellular vesicles containing the S protein induced an increased expression of proinflammatory genes compared to plain extracellular vesicles whereas the opposite was observed in bone-marrow-derived macrophages. These findings reveal the immunomodulatory potential of L. tarentolae extracellular vesicles and provide a proof of concept that they can be used as adjuvant in the context of dendritic cell stimulation.

Type de document:	Article
Mots-clés libres:	Leishmania; SARS-CoV2; Spike protein; dendritic cells; extracellular vesicles; macrophages
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	20 janv. 2025 03:49
Dernière modification:	20 janv. 2025 03:49
URI:	https://espace.inrs.ca/id/eprint/16265

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