Adaptation of a Model Spike Aptamer for Isothermal Amplification-Based Sensing

Yurdusev, Emre; Trahan, Pierre-Luc et Perreault, Jonathan ORCID: https://orcid.org/0000-0003-4726-6319 (2024). Adaptation of a Model Spike Aptamer for Isothermal Amplification-Based Sensing Sensors , vol. 24 , nº 21. pp. 1-15. DOI: 10.3390/s24216875.

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Résumé

Isothermal amplification (IA) techniques like rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) have gained significant attention in recent years due to their ability to rapidly amplify DNA or RNA targets at a constant temperature without the need for complex thermal cycling equipment. Such technologies, combined with colorimetric systems rendering visual confirmation of the amplification event, are ideal for the development of point-of-need detection methods suitable for field settings where access to specialized laboratory equipment is limited. The utility of these technologies, thus far limited to DNA and RNA targets, could be broadened to a wide range of targets by using aptamers. Composed of DNA or RNA themselves, aptamers can bind to substances, including proteins, metabolites, and inorganic substances. Their nucleic acid nature can potentially allow them to serve as a bridge, extending the reach of DNA/RNA-centric technologies to the broader molecular world. Indeed, the change in aptamer conformation occurring during ligand interaction can be used to elaborate ligand-responding RCA or LAMP templates. By using an existing aptamer targeting SARS-CoV-2 Spike protein as a model, we explored the possibility of establishing ligand-responsive IA systems. Our study used aptamers with simple sequence modifications as templates in LAMP assays and hyperbranched RCA (HRCA) by exploiting the dynamic nature of the model aptamer to trigger these IA systems. Importantly, our work uniquely demonstrates that this aptamer’s dynamic response to ligand binding can regulate both RCA and LAMP processes. This novel approach of using aptamer conformational changes to trigger LAMP paves the way for new aptamer-based detection assays. Our system detects 50 nM of Spike protein, with LAMP occurring within 30 min in the presence of Spike. The colorimetric readout showed clear results, allowing for the detection of Spike protein presence.

Type de document:	Article
Mots-clés libres:	Aptamer; isothermal amplification; hyperbranched rolling circle amplification (HRCA); loop-mediated isothermal amplification (LAMP); SARS-CoV-2; Spike protein; colorimetric detection; point-of-care test; allostery; structure dynamics
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	18 nov. 2024 06:44
Dernière modification:	18 nov. 2024 06:44
URI:	https://espace.inrs.ca/id/eprint/16175

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