Pinatel, Helene; Brien, Marie-Eve; Barry, Amadou 
ORCID: https://orcid.org/0000-0003-3396-1724; Delbès, Géraldine ORCID: https://orcid.org/0000-0002-9169-1075; Girard, Sylvie; Chatel-Chaix, Laurent ORCID: https://orcid.org/0000-0002-7390-8250 et Vaillancourt, Cathy ORCID: https://orcid.org/0000-0003-0543-6244
(2024).
Effects of SARS-CoV-2 Infection During Pregnancy on Protein and Gene Expression in the Human Placenta
In: Annual Conference of the International Federation of Placenta Associations, September, 2nd - 6th, 2024, Montréal, Canada.
Résumé
Objectives: The effect of an infection by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), the virus that induces COVID-19 disease,
during pregnancy on the placenta remain poorly understood. Studies have
shown the presence of SARS-CoV-2 in human placentas following infection
during pregnancy as well as the expression of ACE2 receptor and TMPRSS2
co-receptor of SARS-CoV-2 virus in the trophoblasts. Inflammatory and
structural abnormalities have also been observed in placentas from
infected pregnant persons, but the underlying mechanisms are still unknown. Our hypothesis is that a maternal infection during pregnancy with
SARS-CoV-2 alters the development and functions of the placenta. The aim
of our study is to identify the mechanisms involved.
Methods: Placenta sample tissues were collected from 43 pregnant persons infected during their pregnancy and 27 non-infected controls, between March 2020 and June 2021 at CHUSJ (Montreal, Canada). Each was
tested by RTqPCR for the presence of SARS-CoV-2 E-coding RNA and
analysed by mass spectrometry and RNA sequencing (Illumina
Technology).
Results: Our data show that none of the placenta samples exhibit SARSCoV-2 infection footprint at delivery. A preliminary analysis of proteomics
data shows that of the 1272 proteins identified no major difference
(padj˂0.05; FC 2) was detected between the two groups. More in-depth
proteomic and transcriptomic analyzes depending on the stage of infection
and phenotypic data of delivery (sex of the fetus, type of delivery, …) are in
progress. The analysis of the gene expression in the infected group versus
the control group is still ongoing.
Conclusion: This study will allow a better understanding of the effect of
SARS-CoV-2 infection during pregnancy on the placenta at a cohort level.
| Type de document: | Document issu d'une conférence ou d'un atelier |
|---|---|
| Informations complémentaires: | Placenta 154: E58 Meeting AbstractP2.38 Affiche scientifique |
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 05 nov. 2024 22:12 |
| Dernière modification: | 05 nov. 2024 22:12 |
| URI: | https://espace.inrs.ca/id/eprint/16105 |
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