The bacterial strains JAM1(T) and GP59 of the species Methylophaga nitratireducenticrescens differ in their expression profiles of denitrification genes in oxic and anoxic cultures

Lestin, Livie et Villemur, Richard ORCID: https://orcid.org/0000-0001-9768-8937 (2024). The bacterial strains JAM1(T) and GP59 of the species Methylophaga nitratireducenticrescens differ in their expression profiles of denitrification genes in oxic and anoxic cultures PeerJ , vol. 12 , nº e18361. pp. 1-25. DOI: 10.7717/peerj.18361.

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Résumé

BACKGROUND:

Strain JAM1(T) and strain GP59 of the methylotrophic, bacterial species Methylophaga nitratireducenticrescens were isolated from a microbial community of the biofilm that developed in a fluidized-bed, methanol-fed, marine denitrification system. Despite of their common origin, both strains showed distinct physiological characters towards the dynamics of nitrate ( NO3- ) reduction. Strain JAM1(T) can reduce NO3- to nitrite ( NO2- ) but not NO2- to nitric oxide (NO) as it lacks a NO-forming NO2- reductase. Strain GP59 on the other hand can carry the complete reduction of NO3- to N(2). Strain GP59 cultured under anoxic conditions shows a 24-48h lag phase before NO3- reduction occurs. In strain JAM1(T) cultures, NO3- reduction begins immediately with accumulation of NO2- . Furthermore, NO3- is reduced under oxic conditions in strain JAM1(T) cultures, which does not appear in strain GP59 cultures. These distinct characters suggest differences in the regulation pathways impacting the expression of denitrification genes, and ultimately growth.

METHODS:

Both strains were cultured under oxic conditions either with or without NO3- , or under anoxic conditions with NO3- . Transcript levels of selected denitrification genes (nar1 and nar2 encoding NO3- reductases, nirK encoding NO2- reductase, narK12f encoding NO3- / NO2- transporter) and regulatory genes (narXL and fnr) were determined by quantitative reverse transcription polymerase chain reaction. We also derived the transcriptomes of these cultures and determined their relative gene expression profiles.

RESULTS:

The transcript levels of nar1 were very low in strain GP59 cultured under oxic conditions without NO3- . These levels were 37 times higher in strain JAM1(T) cultured under the same conditions, suggesting that Nar1 was expressed at sufficient levels in strain JAM1(T) before the inoculation of the oxic and anoxic cultures to carry NO3- reduction with no lag phase. Transcriptomic analysis revealed that each strain had distinct relative gene expression profiles, and oxygen had high impact on these profiles. Among denitrification genes and regulatory genes, the nnrS3 gene encoding factor involved in NO-response function had its relative gene transcript levels 5 to 10 times higher in strain GP59 cultured under oxic conditions with NO3- than those in both strains cultured under oxic conditions without NO3- . Since NnrS senses NO, these results suggest that strain GP59 reduced NO3- to NO under oxic conditions, but because of the oxic environment, NO is oxidized back to NO3- by flavohemoproteins (NO dioxygenase; Hmp), explaining why NO3- reduction is not observed in strain GP59 cultured under oxic conditions.

CONCLUSIONS:

Understanding how these two strains manage the regulation of the denitrification pathway provided some clues on how they response to environmental changes in the original biofilm community, and, by extension, how this community adapts in providing efficient denitrifying activities.

Type de document:	Article
Mots-clés libres:	Denitrification; Gene expression; Methylophaga; Nitrate reduction dynamics; RT-qPCR; Species sub-population; Transcriptome
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	06 nov. 2024 06:02
Dernière modification:	06 nov. 2024 06:02
URI:	https://espace.inrs.ca/id/eprint/16103

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