Cyr, Daniel G. 
ORCID: https://orcid.org/0000-0002-6566-783X; Adam, Cécile; Dufresne, Julie et Gregory, Mary
(2024).
Regulation of the gap junction interplay during postnatal development in the rat epididymis
Cell and Tissue Research
, vol. ahead
.
DOI: 10.1007/s00441-024-03919-1.
(Sous Presse)
Résumé
During postnatal development of the rat epididymis, a change in the expression of gap junction proteins, or connexins (Cxs), occurs, in which Gjb2 (Cx26) and Gja1 (Cx43) levels in the proximal epididymis are decreased, while Gjb1 (Cx32), Gjb4 (Cx30.3) and Gjb5 (Cx31.1) levels increase. The mechanism(s) responsible for the switch in Cx expression is unknown. The aim of this study is to identify the mechanisms responsible for the decrease in GJB2 protein levels and the increase in other Cxs during postnatal development. Results indicate that decreased Gjb2 expression for 48 h does not alter the expression of other Cxs in RCE-1 principal cells, suggesting a lack of compensatory expression. Sequence analysis of both Gjb2 and Gjb1 promoters identified common multiple response elements to steroid hormones. Using RCE-1 cells, we observed that dexamethasone increased Gjb2 mRNA levels by twofold after 48 h, while estradiol had no effect. Orchidectomy in rats resulted in a significant increase in GJB2 and decreased GJB1 in the caput and corpus epididymidis. Changes in Cxs protein levels were prevented by testosterone in orchidectomized rats. Similar results were observed in the prostate, another androgen-receptive organ. LNCaP cells, which are androgen-responsive, showed that exogenous dihydrotestosterone (DHT) decreased Gjb2 mRNA levels by approximately 50% concomitant with a 1.5-fold increase in Gjb1 levels. Using a GJB1 promoter construct we showed that DHT could induce transactivation of the luciferase transgene, while transactivation of two GJB2 promoters were unaltered. Results indicate that androgens and glucocorticoids regulate the expression of epididymal Cxs.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Androgen; Connexins; Development; Epididymis; Gap junctions; Gene expression |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 20 oct. 2024 10:51 |
| Dernière modification: | 20 oct. 2024 10:51 |
| URI: | https://espace.inrs.ca/id/eprint/16015 |
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