Honeybee gut bacterial strain improved survival and gut microbiota homeostasis in Apis mellifera exposed in vivo to clothianidin.

El Khoury, Sarah ORCID: https://orcid.org/0000-0003-1846-1043; Gauthier, Jeff ORCID: https://orcid.org/0000-0002-2523-2698; Mercier, Pierre Luc; Moïse, Stéphane; Giovenazzo, Pierre et Derome, Nicolas ORCID: https://orcid.org/0000-0002-2509-6104 (2024). Honeybee gut bacterial strain improved survival and gut microbiota homeostasis in Apis mellifera exposed in vivo to clothianidin. Microbiology Spectrum , vol. 12 , nº 10. e00578-24. DOI: 10.1128/spectrum.00578-24.

PDF - Version publiée Document sous embargo jusqu'à 27 mars 2025. Télécharger (19MB)

Résumé

Pesticides are causing honeybee mortality worldwide. Research carried out on honeybees indicates that application of pesticides has a significant impact on the core gut community, which ultimately leads to an increase in the growth of harmful pathogens. Disturbances caused by pesticides also affect the way bacterial members interact, which results in gut microbial dysbiosis. Administration of beneficial microbes has been previously demonstrated to be effective in treating or preventing disease in honeybees. The objective of this study was to measure under in vivo conditions the ability of two bacterial strains (the Enterobacter sp. and Pantoea sp.) isolated from honeybee gut to improve survival and mitigate gut microbiota dysbiosis in honeybees exposed to a sublethal clothianidin dose (0.1 ppb). Both gut bacterial strains were selected for their ability to degrade clothianidin in vitro regardless of their host–microbe interaction characteristics (e.g., beneficial, neutral, or harmful). To this end, we conducted cage trials on 4- to 6-day-old newly emerging honeybees. During microbial administration, we jointly monitored the taxonomic distribution and activity level of bacterial symbionts quantifying 16S rRNA transcripts. First, curative administration of the Pantoea sp. strain significantly improved the survival of clothianidin-exposed honeybees compared to sugar control bees (i.e., supplemented with sugar [1:1]). Second, curative administration of the Enterobacter sp. strain significantly mitigated the clothianidininduced dysbiosis observed in the midgut structural network, but without improving survival.

Type de document:	Article
Mots-clés libres:	honeybee; clothianidin; microbiota; dysbiosis; beneficial microbes
Centre:	Centre Eau Terre Environnement
Date de dépôt:	12 nov. 2024 16:54
Dernière modification:	12 nov. 2024 16:54
URI:	https://espace.inrs.ca/id/eprint/16013

Gestion Actions (Identification requise)

Modifier la notice