Erasure of DNA methylation in rat fetal germ cells is sex-specific and sensitive to maternal high-fat diet

El Omri-Charai, Rizlane; Rwigemera, Arlette; Gilbert, Isabelle; Langford, Alexandra; Robert, Claude; Sloboda, Deborah; McGraw, Serge et Delbès, Géraldine ORCID: https://orcid.org/0000-0002-9169-1075 (2024). Erasure of DNA methylation in rat fetal germ cells is sex-specific and sensitive to maternal high-fat diet Journal of Developmental Origins of Health and Disease , vol. 15 , nº e19. pp. 1-6. DOI: 10.1017/S2040174424000230.

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Résumé

In mammals, DNA methylation (DNAme) erasure and reinstatement during embryo development and germline establishment are sensitive to the intrauterine environment. Maternal intake of a high-fat diet (HFD), associated with excessive gestational weight gain, has transgenerational effects on offspring health, which may be mediated by changes in DNAme in the germline. Here, we tested the impact of a maternal HFD on embryonic germline DNAme erasure using a rat strain that expresses green fluorescent protein specifically in germ cells. DNAme was analysed by methyl-seq capture in germ cells collected from male and female F1 gonads at gestational day 16. Our data show that although HFD induced global hypomethylation in both sexes, DNAme erasure in female germ cells was more advanced compared to male germ cells. The delay in DNAme erasure in males and the greater impact of HFD suggest that male germ cells are more vulnerable to alterations by exogenous factors.

Type de document:	Article
Mots-clés libres:	DNA methylation; germ cells; maternal high-fat diet; rat; reprogramming
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	29 sept. 2024 05:32
Dernière modification:	29 sept. 2024 05:32
URI:	https://espace.inrs.ca/id/eprint/15984

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