Sofeo, Naazneen 
ORCID: https://orcid.org/0000-0001-5724-3394; Toi, Min Gin ORCID: https://orcid.org/0009-0004-4466-6274; Ee, En Qi Grace; Ng, Jing Yang ORCID: https://orcid.org/0009-0000-9032-492X; Busran, Coleen Toledo; Lukito, Benedict Ryan; Thong, Aaron ORCID: https://orcid.org/0000-0003-1852-4067; Hermansen, Christian ORCID: https://orcid.org/0000-0002-2740-780X; Peterson, Eric Charles ORCID: https://orcid.org/0000-0003-1513-6106; Glitsos, Renata et Arumugam, Prakash
(2024).
Sustainable production of lipids from cocoa fatty acid distillate fermentation driven by adaptive evolution in Yarrowia lipolytica.
Bioresource Technology
, vol. 394
.
p. 130302.
DOI: 10.1016/j.biortech.2024.130302.
Résumé
Single cell oil production using oleaginous yeasts is a promising alternative to animal and plant-derived lipids. But substrate costs for microbial fermentation are a major bottleneck. Using side streams as alternative to substrates like glucose, for growing yeast, is a potential cost-effective solution. By combining a previously reported process of growing yeasts on a solid cocoa fatty acid distillate side stream with adaptive evolution techniques, the growth of oleaginous yeast Yarrowia lipolytica was improved by 2-fold. The lipid titre was also boosted by more than 3-fold. Using transcriptomics, key genes were identified that are possibly involved in tailoring of lipid composition, side stream utilisation and enhancement of lipid titres. Candidate genes were also identified that might enable efficient growth and utilization of fatty acids and triacylglycerides found in cocoa fatty acid distillate. In summary, this research has improved the understanding of side stream utilisation for lipid production in oleaginous yeast.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Single Cell Oil (SCO); waste lipids; rna-seq; oleaginous yeasts; valorization |
| Centre: | Centre Eau Terre Environnement |
| Date de dépôt: | 31 juill. 2024 20:04 |
| Dernière modification: | 31 juill. 2024 20:04 |
| URI: | https://espace.inrs.ca/id/eprint/15484 |
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