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Effect of the addition of CMC on the aggregation behaviour of proteins

Yu, Hanling; Sabato, Susy Frey; D'Aprano, Giuseppe et Lacroix, Monique ORCID logoORCID: https://orcid.org/0000-0002-2042-4033 (2004). Effect of the addition of CMC on the aggregation behaviour of proteins Radiation Physics and Chemistry , vol. 71 , nº 1-2. pp. 129-133. DOI: 10.1016/j.radphyschem.2004.04.003.

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Résumé


The effect of carboxymethylcellulose (CMC) on the aggregation of formulation based on calcium caseinate, commercial whey protein (WPC), and a 1:1 mixture of soy protein isolate (SPI) and whey protein isolate (WPI) was investigated. Protein aggregation could be observed upon addition of CMC, as demonstrated by size-exclusion chromatography. This aggregation behaviour was enhanced by means of physical treatments, such as heating at 90°C for 30min or gamma-irradiation at 32kGy. A synergy resulted from the combination of CMC to gamma-irradiation in Caseinate/CMC and SPI/WPI/CMC formulations. Furthermore, CMC prevented precipitation in irradiated protein solutions for a period of more than 3 months at 4°C.

Type de document: Article
Mots-clés libres: Carboxymethylcellulose; Proteins; Aggregation; Gamma-irradiation
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 25 déc. 2024 16:31
Dernière modification: 25 déc. 2024 16:31
URI: https://espace.inrs.ca/id/eprint/15290

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