Winberg, Martin E.; Holm, Asa; Särndahl, Eva; Vinet, Adrien F.; Descoteaux, Albert 
ORCID: https://orcid.org/0000-0002-0633-5309; Magnusson, Karl-Eric; Rasmusson, Birgitta et Lerm, Maria
(2009).
Leishmania donovani lipophosphoglycan inhibits phagosomal maturation via action on membrane rafts
Microbes and Infection
, vol. 11
, nº 2.
pp. 215-222.
DOI: 10.1016/j.micinf.2008.11.007.
Résumé
Lipophosphoglycan (LPG), the major surface glycoconjugate on Leishmania donovani promastigotes, is crucial for the establishment of infection inside macrophages. LPG comprises a polymer of repeating Galβ1,4Manα-PO4 attached to a lysophosphatidylinositol membrane anchor. LPG is transferred from the parasite to the host macrophage membrane during phagocytosis and induces periphagosomal F-actin accumulation correlating with an inhibition of phagosomal maturation. The biophysical properties of LPG suggest that it may be intercalated into membrane rafts of the host-cell membrane. The aim of this study was to investigate if the effects of LPG on phagosomal maturation are mediated via action on membrane rafts. We show that LPG accumulates in rafts during phagocytosis of L. donovani and that disruption of membrane rafts abolished the effects of LPG on periphagosomal F-actin and phagosomal maturation, indicating that LPG requires intact membrane rafts to manipulate host-cell functions. We conclude that LPG associates with membrane rafts in the host cell and exert its actions on host-cell actin and phagosomal maturation through subversion of raft function. © 2008 Elsevier Masson SAS. All rights reserved.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Leishmania; Lipophosphoglycan; Membrane rafts; Phagosomal maturation; Actin |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 21 juill. 2024 19:57 |
| Dernière modification: | 21 juill. 2024 19:57 |
| URI: | https://espace.inrs.ca/id/eprint/15287 |
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