Interleukin (IL)-4 induces leukocyte infiltration in vivo by an indirect mechanism

Ratthé, Claude; Ennaciri, Jamila; Garcês Gonçalves, David M; Chiasson, Sonia et Girard, Denis ORCID: https://orcid.org/0000-0002-3342-5027 (2009). Interleukin (IL)-4 induces leukocyte infiltration in vivo by an indirect mechanism Mediators of Inflammation , vol. 2009 , nº 193970. pp. 1-10. DOI: 10.1155/2009/193970.

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Résumé

Interleukin (IL)-4 is a cytokine known mainly for its anti-inflammatory activity. Using the in vivo murine air pouch model, we found that IL-4 significantly increased the number of leukocytes after 9 hours of treatment, consisting mainly of neutrophil (60%) and monocytic (40%) cell populations. Using an antibody array, we found that the expression of several analytes (predominantly CCL2) was increased by IL-4 before the arrival of leukocytes. The IL-4-induced expression of CCL-2 was confirmed by ELISA. Air pouch resident lining cells were harvested and were found to express IL-4Ralpha. CCL2 mRNA expression was monitored in lining cells, cells isolated from the air pouch skin, in RAW264.7 macrophage and in epithelial Mode-K cells and its expression was increased in response to IL-4 in all conditions. We conclude that IL-4 can attract leukocytes in vivo by an indirect mechanism involving the production of several analytes by, at least, resident cells.

Type de document:	Article
Mots-clés libres:	-
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	01 juill. 2024 03:29
Dernière modification:	01 juill. 2024 03:29
URI:	https://espace.inrs.ca/id/eprint/15101

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