Rashidan, Kianoush Khajeh; Nassoury, Nasha; Merzouki, Abderrazzak et Guertin, Claude 
ORCID: https://orcid.org/0000-0002-0710-6970
(2002).
Identification and characterization of a putative baculoviral transcriptional factor IE-1 from Choristoneura fumiferana granulovirus
Journal of Biochemistry and Molecular Biology
, vol. 35
, nº 6.
pp. 553-561.
DOI: 10.5483/bmbrep.2002.35.6.553.
Résumé
A gene that encodes a protein homologue to baculoviral IE-1 was identified and sequenced in the genome of the Choristoneura fumiferana granulovirus (ChfuGV). The gene has an 1278 nucleotide (nt) open-reading frame (ORF) that encodes 426 amino acids with an estimated molecular weight of 50.33 kDa. At the nucleotide level, several cis-acting regulatory elements were detected within the promoter region of the ie-1 gene of ChfuGV along with other studied granuloviruses (GVs). Two putative CCAAT elements were detected within the noncoding leader region of this gene; one was located on the opposite strand at -92 and the other at -420 nt from the putative start triplet. Two baculoviral late promoter motifs (TAAG) were also detected within the promoter region of the ie-1 gene of ChfuGV. A single polyadenylation signal, AATAAA, was located 18nt downstream of the putative translational stop codon of ie-1 from ChfuGV. At the protein level, the amino acid sequence data that was derived from the nucleotide sequence in ChfuGV IE-1 was compared to those of the Cydia pomonella granulovirus (CpGV), Xestia c-nigrum granulovirus (XcGV) and Plutella xylostella granulovirus (PxGV). The C-terminal regions of the granuloviral IE-1 sequences appeared to be more conserved when compared to the N-terminal regions. A domain, similar to the basic helix-loop-helix like (bHLH-like) domain in NPVs, was detected at the C-terminal region of IE-1 from ChfuGV (residues 387 to 414). A phylogenetic tree for baculoviral IE-1 was constructed using a maximum parsimony analysis. A phylogenetic estimation demonstrates that ChfuGV IE-1 is most closely related to that of CpGV.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Choristoneura fumiferana granulovirus; ie-1; Phylogeny; Protein analysis; Transcriptional factor |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 07 mars 2025 21:37 |
| Dernière modification: | 07 mars 2025 21:37 |
| URI: | https://espace.inrs.ca/id/eprint/15099 |
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