Rashidan, Kianoush Khajeh; Nassoury, Nasha; Giannopoulos, Paresa N. et Guertin, Claude 
ORCID: https://orcid.org/0000-0002-0710-6970
(2005).
Transcription, translation, and immunolocalization of ODVP-6E/ODV-E56 and p74 proteins: Two highly conserved ODV-associated envelope proteins of Choristoneura fumiferana granulovirus
Journal of Biochemistry and Molecular Biology
, vol. 38
, nº 1.
pp. 65-70.
DOI: 10.5483/bmbrep.2005.38.1.065.
Résumé
Choristoneura fumiferana granulovirus (ChfuGV) infection results two types of enveloped virions: Occlusion-derived virus (ODV) and budded virus (BV). Structural proteins ODVP-6E/ODV-E56 and p74 are two major conserved ODV-associated proteins that may be involved in the initiation of viral infection cycle in susceptible host insect larvae. This study presents the characterization of ChfuGV odvp-6e/odv-e56 and p74 transcription and translation as well as immunolocalization of these proteins in the occluded ChfuGV virion. Our results revealed that the transcription of odvp-6e/odv-e56 and p74 genes, both, start at 24 hours post infection (h p.i.). Using monospecific polyclonal antibodies made against ODVP-6E/ODV-E56 and p74 we demonstrated that these proteins are both expressed late in infection (24 h p.i.). Immunogold labeling using antisera against ODVP-6E/ODV-E56 and p74 proteins demonstrated that ODVP-6E/ODV-E56 and p74 proteins are both associated with the ODV envelop of ChfuGV.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 11 nov. 2024 20:30 |
| Dernière modification: | 11 nov. 2024 20:30 |
| URI: | https://espace.inrs.ca/id/eprint/15097 |
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