Primiani, Nadia; Gregory, Mary; Dufresne, Julie; Smith, Charles E.; Ye, Lauren Liu; Bartles, James R; Cyr, Daniel G. 
ORCID: https://orcid.org/0000-0002-6566-783X et Hermo, Louis
(2007).
Microvillar size and espin expression in principal cells of the adult rat epididymis are regulated by androgens
Journal of Andrology
, vol. 28
, nº 5.
pp. 659-669.
DOI: 10.2164/jandrol.107.002634.
Résumé
Principal cells of the epididymis are the most prominent cell type and are noted for an apical cell surface studded with microvilli. The latter contain channel proteins that condition the microenvironment of epididymal lumen and promote sperm maturation; however, the regulation of the structure and integrity of microvilli is not well known. Espins are a family of proteins implicated in microvillar growth. The objectives of this study were to assess the regulation of espin in epididymal principal cells both in vitro and in vivo. Treatment of immortalized rat caput epididymal (RCE) cells with increasing doses of a homogenized testicular extract revealed a dose-dependent increase in the size of microvilli. Reverse transcriptase-polymerase chain reaction (RT-PCR) of adult rat epididymal RNA using espin-specific primers indicated the presence of a band at about 290 base pairs (bp) in all regions. Western blot analysis using affinity-purified espin antibody confirmed the presence of an approximately 110-kDa band in the epididymis, corresponding to espin isoform 1. In adult rats, immunocytochemistry revealed espin expression over principal cells. In orchidectomized rats, espin expression was significantly reduced, whereas ligation of the efferent ducts resulted in a decrease of espin expression but not to the extent of orchidectomy. The fact that espin expression was restored to control levels in orchidectomized rats supplemented with high levels of testosterone indicated that its expression was dependent on androgens and not on other lumicrine factors derived from the testis. Taken together, these data indicate that espin is expressed in the epididymis and is regulated by androgens.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | RT-PCR; immunocytochemistry; RCE cell line; orchidectomy; testicular extract |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 05 nov. 2024 14:21 |
| Dernière modification: | 05 nov. 2024 14:21 |
| URI: | https://espace.inrs.ca/id/eprint/15083 |
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