Identification of hammerhead ribozymes in all domains of life reveals novel structural variations

Perreault, Jonathan ORCID: https://orcid.org/0000-0003-4726-6319; Weinberg, Zasha; Roth, Adam; Popescu, Olivia; Chartrand, Pascal; Ferbeyre, Gerardo et Breaker, Ronald R. (2011). Identification of hammerhead ribozymes in all domains of life reveals novel structural variations PLoS Computational Biology , vol. 7 , nº 5. pp. 1-13. DOI: 10.1371/journal.pcbi.1002031.

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Résumé

Hammerhead ribozymes are small self-cleaving RNAs that promote strand scission by internal phosphoester transfer. Comparative sequence analysis was used to identify numerous additional representatives of this ribozyme class than were previously known, including the first representatives in fungi and archaea. Moreover, we have uncovered the first natural examples of "type II" hammerheads, and our findings reveal that this permuted form occurs in bacteria as frequently as type I and III architectures. We also identified a commonly occurring pseudoknot that forms a tertiary interaction critical for high-speed ribozyme activity. Genomic contexts of many hammerhead ribozymes indicate that they perform biological functions different from their known role in generating unit-length RNA transcripts of multimeric viroid and satellite virus genomes. In rare instances, nucleotide variation occurs at positions within the catalytic core that are otherwise strictly conserved, suggesting that core mutations are occasionally tolerated or preferred.

Type de document:	Article
Informations complémentaires:	document e1002031
Mots-clés libres:	-
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	04 avr. 2024 16:02
Dernière modification:	04 avr. 2024 16:02
URI:	https://espace.inrs.ca/id/eprint/15056

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