Patrick, Casey B.; Krzywkowski, Pascale; Ramassamy, Charles 
ORCID: https://orcid.org/0000-0002-3252-5878; Poirier, Judes; Rapoport, Stanley Isaac et Murphy, Eric J.
(2000).
Phospholipase A2 activity is decreased selectively in the hippocampus of aged apolipoprotein E deficient mice
Neuroscience Letters
, vol. 288
, nº 3.
pp. 211-214.
DOI: 10.1016/s0304-3940(00)01240-4.
Résumé
Because apolipoprotein E (ApoE) deficient mice have cognitive deficits (Neurosci. Lett., 199 (1995) 1-4; Neuroscience, 92 (1999) 1273-1286; Brain Res., 752 (1997) 189-196) that may involve decreased phospholipase A(2) (PLA(2)) activity (Neuroscience, 92 (1999) 1273-1286), striatal, hippocampal, and parieto-temporal PLA(2) activity was measured in cytosol from 3 and 20-month-old ApoE deficient and control mice. Samples were homogenized and cytosol prepared by ultracentrifugation. PLA(2) activity in each cytosolic fraction was measured in triplicate using a continuous fluorometric assay (J. Neurosci. Methods (2000) in press). In 3-month-old animals, there was a trend for decreased hippocampal PLA(2) activity between groups. In 20-month-old animals, hippocampal PLA(2) activity was significantly (P=0.0304) decreased nearly 20% in ApoE deficient mice as compared to age-matched control mice. No differences were found in other brain regions, although activity in the striatal samples were nearly 65% less than in the other two regions.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Long term potentiation; Apolipoprotein E; Phospholipase A2; Hippocampus; Knockout mice; Aging; Alzheimer disease; Arachidonic acid |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 14 avr. 2025 14:29 |
| Dernière modification: | 14 avr. 2025 14:29 |
| URI: | https://espace.inrs.ca/id/eprint/15040 |
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