Mak, Paul; Palant, Olga; Labonté, Patrick 
ORCID: https://orcid.org/0000-0001-7262-3125 et Plotch, Steve
(2001).
Reconstitution of hepatitis C virus protease activities in yeast
FEBS Letters
, vol. 503
, nº 1.
pp. 13-18.
DOI: 10.1016/s0014-5793(01)02678-3.
Résumé
The hepatitis C virus (HCV) protease genes (NS2/3 and NS3) were expressed in yeast with their natural substrates fused to a ligand-dependent transcriptional activator, the retinoic acid receptor (RARbeta). RARbeta can activate transcription in yeast cells in response to retinoic acids. We hypothesized that cis-cleavage at the NS2-3 or NS3-4A junctions by the appropriate HCV proteases would release RARbeta, thereby activating transcription of a reporter gene. Our results from Western blot analyses and reporter gene activation indicate that the wild-type NS2/3 and NS3 enzymes are catalytically active in yeast cells, whereas mutations in the catalytic domain of NS2(C993V) and NS3(S1165A) lead to inactive enzymes. We conclude that HCV NS2/3 and NS3 protease activities can be reconstituted in yeast.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | HCV protease; Retinoic acid receptor; Saccharomyces cerevisiae |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 11 avr. 2025 21:29 |
| Dernière modification: | 11 avr. 2025 21:29 |
| URI: | https://espace.inrs.ca/id/eprint/14941 |
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