Lymberopoulos, Maria H. et Pearson, Angela 
ORCID: https://orcid.org/0000-0002-5997-2846
(2007).
Involvement of UL24 in herpes-simplex-virus-1-induced dispersal of nucleolin
Virology
, vol. 363
, nº 2.
pp. 397-409.
DOI: 10.1016/j.virol.2007.01.028.
Résumé
UL24 of herpes simplex virus 1 is important for efficient viral replication, but its function is unknown. We generated a recombinant virus, vHA-UL24, encoding UL24 with an N-terminal hemagglutinin tag. By indirect immunofluorescence at 9 h post-infection (hpi), we detected HAUL24 in nuclear foci and in cytoplasmic speckles. HA-UL24 partially co-localized with nucleolin, but not with ICP8 or coilin, markers for nucleoli, viral replication compartments, and Cajal bodies respectively. HA-UL24 staining was often juxtaposed to that of another nucleolar protein, fibrillarin. Analysis of HSV-1-induced nucleolar modifications revealed that by 18 hpi, nucleolin staining had dispersed, and fibrillarin staining went from clusters of small spots to a few separate but prominent spots. Fibrillarin redistribution appeared to be independent of UL24. In contrast, cells infected with a UL24-deficient virus retained foci of nucleolin staining. Our results demonstrate involvernent of UL24 in dispersal of nucleolin during infection.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Nucleolus; B23; HSV-1 UL24; Nuclear egress |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 04 nov. 2024 20:26 |
| Dernière modification: | 04 nov. 2024 20:26 |
| URI: | https://espace.inrs.ca/id/eprint/14927 |
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