Lymberopoulos, Maria H.; Bourget, Amélie; Ben Abdeljelil, Nawel et Pearson, Angela 
ORCID: https://orcid.org/0000-0002-5997-2846
(2011).
Involvement of the UL24 protein in herpes simplex virus 1-induced dispersal of B23 and in nuclear egress
Virology
, vol. 412
, nº 2.
pp. 341-348.
DOI: 10.1016/j.virol.2011.01.016.
Résumé
UL24 of herpes simplex virus 1 (HSV-1) is widely conserved within the Herpesviridae family. Herein, we tested the hypothesis that UL24, which we have previously shown to induce the redistribution of nucleolin, also affects the localization of the nucleolar protein B23. We found that HSV-1-induced dispersal of B23 was dependent on UL24. The conserved N-terminal portion of UL24 was sufficient to induce the redistribution of B23 in transient transfection assays. Mutational analysis revealed that the endonuclease motif of UL24 was important for B23 dispersal in both transfected and infected cells. Nucleolar protein relocalization during HSV-1 infection was also observed in non-immortalized cells. Analysis of infected cells by electron microscopy revealed a decrease in the ratio of cytoplasmic versus nuclear viral particles in cells infected with a UL24-deficient strain compared to KOS-infected cells. Our results suggest that UL24 promotes nuclear egress of nucleocapsids during HSV-1 infection, possibly though effects on nucleoli. (C)
| Type de document: | Article |
|---|---|
| Informations complémentaires: | article en accès libre |
| Mots-clés libres: | Nucleolus; B23; HSV-1; UL24; Nuclear egress |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 27 mars 2024 15:21 |
| Dernière modification: | 27 mars 2024 15:21 |
| URI: | https://espace.inrs.ca/id/eprint/14925 |
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