Letowski, Jaroslaw; Juteau, Pierre; Villemur, Richard 
ORCID: https://orcid.org/0000-0001-9768-8937; Duckett, Marie-France; Beaudet, Réjean; Lépine, François et Bisaillon, Jean-Guy
(2001).
Separation of a phenol carboxylating organism from a two-member, strict anaerobic co-culture
Canadian Journal of Microbiology
, vol. 47
, nº 5.
pp. 373-381.
DOI: 10.1139/w01-023.
Résumé
In a culture converting phenol to benzoic acid under anaerobic conditions and previously described as being constituted of only a Clostridium-like strain 6, another bacterium (strain 7) was observed. Each organism was enriched by centrifugation on a Percoll gradient. Strain 6 was purified by dilution and plating. Strain 7 did not grow on solid media, but a strain 7 culture, cleared of strain 6, was obtained by subculturing in the presence of ampicillin and by dilution. In fresh medium, phenol was transformed by the reconstituted co-culture but not by each strain alone. In a supernatant from a co-culture or from a strain 6 culture, strain 7 alone transformed phenol but not strain 6. Maintenance of an active strain 7 in fresh medium instead of co-culture supernatant became possible when phenol was replaced by 4-hydroxybenzoate (4-OHB), which is decarboxylated to phenol before being transformed to benzoate. Even with 4-OHB, the use of co-culture (or strain 6 culture) supernatant resulted in faster transformation activity and growth rate. A phylogenetic analysis placed strain 7 in a cluster of uncultivated or nonisolated bacteria (92-96% homology). Strain 7 is also related to Desulfotomaculum, Desulfitobacterium, Desulfosporosinus, Moorella, and Sporotomaculum genera (87-92% homology).
| Type de document: | Article |
|---|---|
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 11 avr. 2025 21:24 |
| Dernière modification: | 11 avr. 2025 21:24 |
| URI: | https://espace.inrs.ca/id/eprint/14900 |
Gestion Actions (Identification requise)
 |
Modifier la notice |