Lavastre, Valérie; Binet, François; Moisan, Eliane; Chiasson, Sonia et Girard, Denis 
ORCID: https://orcid.org/0000-0002-3342-5027
(2007).
Viscum album agglutinin-I induces degradation of cytoskeletal proteins in leukaemia PLB-985 cells differentiated toward neutrophils: Cleavage of non-muscle myosin heavy chain-IIA by caspases
British Journal of Haematology
, vol. 138
, nº 4.
pp. 545-554.
DOI: 10.1111/j.1365-2141.2007.06692.x.
Résumé
The role of the anti-cancer agent Viscum album agglutinin-I (VAA-I) in leukaemia PLB-985 cells differentiated toward a neutrophil-like phenotype by dimethylsulphoxide (PLB-985D) has never been studied. This study investigated whether or not VAA-I can induce cytoskeletal breakdown in PLB-985D cells, as previously observed in undifferentiated PLB-985 cells. VAA-I was found to induce apoptosis in PLB-985D cells, as assessed by cytology and by degradation of gelsolin, an event known to occur via caspase-3 activation. VAA-I induced cytoskeletal breakdown based on the disruption of the F-actin network and cleavage of paxillin, vimentin and lamin B(1). In addition, we demonstrated, for the first time, that non-muscle myosin heavy chain IIA (NMHC-IIA) was cleaved by VAA-I treatment. Degradation of NMHC-IIA was reversed by the pan caspase inhibitor z-VAD-fmk in PLB-985D cells and neutrophils. However, unlike lamin B(1), no NMHC-IIA was detected on the cell surface of apoptotic neutrophils. In conclusion, PLB-985D cells responded in a similar manner to neutrophils regarding the degradation of the tested cytoskeletal. Therefore, PLB-985D cells may provide a suitable substitute for neutrophils in screening experiments, preventing extensive neutrophil cell isolation.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Leukaemia cells; neutrophils; cytoskeleton; caspases; myosin |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 04 nov. 2024 17:25 |
| Dernière modification: | 04 nov. 2024 17:25 |
| URI: | https://espace.inrs.ca/id/eprint/14865 |
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