Lanthier, Martin; Villemur, Richard ORCID: https://orcid.org/0000-0001-9768-8937; Lépine, François; Bisaillon, Jean-Guy et Beaudet, Réjean
(2001).
Geographic distribution of Desulfitobacterium frappieri PCP-1 and Desulfitobacterium spp. in soils from the province of Quebec, Canada
FEMS Microbiology Ecology
, vol. 36
, nº 2-3.
pp. 185-191.
DOI: 10.1016/S0168-6496(01)00132-5.
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Résumé
The presence of indigenous Desulfitobacterium species in 44 soil samples taken from various sites in the southern part of the province of Quebec (Canada) and four from locations outside Quebec was investigated. Twenty-four of these soils were sampled from contaminated industrial sites. Indigenous Desulfitobacterium bacteria from soil samples were enriched by cultivation in anaerobic soil slurry culture. Total DNA was then extracted from these slurries and polymerase chain reaction (PCR) amplifications were performed with primers targeting 16S ribosomal RNA gene sequences of Desulfitobacterium spp. and of Desulfitobacterium frappieri PCP-1. A positive PCR signal was obtained in 31 soil slurry cultures. Resolution of single-strand DNAs of some of the PCR products by a single-strand conformational polymorphism protocol suggests that more than one species of Desulfitobacterium were present in the corresponding slurry cultures. These results suggest that Desulfitobacterium are ubiquitous in soils in the province of Quebec, especially in soils from the St. Lawrence valley and the southern part of the province.
Type de document: | Article |
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Mots-clés libres: | Dehalogenation; Desulfitobacterium; Microbial ecology; Polymerase chain reaction; Single-strand gel polymorphism |
Centre: | Centre INRS-Institut Armand Frappier |
Date de dépôt: | 07 mars 2025 21:26 |
Dernière modification: | 07 mars 2025 21:30 |
URI: | https://espace.inrs.ca/id/eprint/14856 |
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