Lanoix, Dave; Beghdadi, Hamza; Lafond, Julie et Vaillancourt, Cathy 
ORCID: https://orcid.org/0000-0003-0543-6244
(2008).
Human placental trophoblasts synthesize melatonin and express its receptors
Journal of Pineal Research
, vol. 45
, nº 1.
pp. 50-60.
DOI: 10.1111/j.1600-079X.2008.00555.x.
Résumé
Although the role of melatonin on fetal development has been the subject of a number of studies, little is known about the function of melatonin in the placenta. We previously showed that melatonin receptors are expressed and are functional in JEG-3 and BeWo cell lines, both in vitro models of human trophoblast. Local synthesis of melatonin in placenta has been proposed, but the human placenta's ability to synthesize melatonin de novo has never been studied. The purpose of this study was to investigate the expression [reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis] and activity (radiometric assay) of melatonin synthesizing enzymes, and characterize the expression of the melatoninergic receptors in human term villous trophoblast. The results show that arylalkylamine N-acetyltransferase and hydroxyindole O-methyltransferase melatonin synthesizing enzymes are expressed and active in villous trophoblast as well as in JEG-3 and BeWo placental choriocarcinoma cells. In addition, immunohistochemical analysis demonstrated the presence of MT1, MT2, and retinoid-related orphan nuclear receptor alpha melatonin receptor proteins in both villous cytotrophoblast and syncytiotrophoblast (STB) as well as in endothelial cells surrounding the fetal capillaries and in the villous mesenchymal core. RT-PCR and western blot analysis in primary cultures of human term trophoblast confirmed the expression of all three melatonin receptors in villous cytotrophoblast and STB cells. This study demonstrates for the first time a local synthesis of melatonin and expression of its receptors in human trophoblasts and strongly suggests a paracrine, autocrine, and/or intracrine role for this indolamine in placental function and development as well as in protection from oxidative stress.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | arylalkylamine N-acetyltransferase; human placenta; hydroxyindole O-methyltransferase; melatonin; MT1-melatonin receptor; MT2-melatonin receptor; retinoid-related orphan nuclear receptor α; trophoblast cells |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 15 août 2024 04:01 |
| Dernière modification: | 15 août 2024 04:01 |
| URI: | https://espace.inrs.ca/id/eprint/14850 |
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