Juteau, Pierre; Côté, Valérie; Duckett, Marie-France; Beaudet, Réjean; Lépine, François; Villemur, Richard 
ORCID: https://orcid.org/0000-0001-9768-8937 et Bisaillon, Jean-Guy
(2005).
Cryptanaerobacter phenolicus gen. nov., sp nov., an anaerobe that transforms phenol into benzoate via 4-hydroxybenzoate
International Journal of Systematic and Evolutionary Microbiology
, vol. 55
, nº Pt 1.
pp. 245-250.
DOI: 10.1099/ijs.0.02914-0.
Résumé
An anaerobic bacterium that transforms phenol and 4-hydroxybenzoate (4-OHB) into benzoate, strain LR7.2(T) was isolated from a culture originating from a mixture of swamp water, sewage sludge, swine waste and soil. Cells of strain LR7.2(T) are Gram-positive short rods (11 x 2 pm) that are electron-dense when observed by electron microscopy. The optimum pH and temperature for growth and transformation activity of 4-OHB are 7(.)5-8(.)0 and 30-37degreesC, respectively. The bacterium does not use sulphate, thiosulphate, nitrate, nitrite, FeCl3, fumarate or arsenate as an electron acceptor. It does not normally use sulphite, although stimulation of growth and 4-OHB transformation activity at a low concentration (up to 2 mM) has been reported previously under different culture conditions. The presence of 4-OHB or phenol is essential for growth; transformation of 4-OHB or phenol into benzoate is used to produce energy for growth. Using [D-6]-phenol, 4-OHB was shown to be an intermediate in the transformation of phenol into benzoate. No spore was observed. The bacterium has a DNA G + C content of 51 mol% and its major membrane fatty acid is anteiso-C-15: 0. The 16S rRNA gene sequence of strain LR7.2T shows only 90 % similarity to its closest relative (Pelotomaculum thermopropionicum). From these results, a new taxon is proposed: Cryptanaerobacter phenolicus gen. nov., sp. nov. The type strain is LR7.2(T) (-ATCC BAA-820(T) =DSM 15808(T)).
| Type de document: | Article |
|---|---|
| Mots-clés libres: | FAME; fatty acid methyl ester; 4-OHB; 4-hydroxybenzoate |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 04 nov. 2024 16:29 |
| Dernière modification: | 04 nov. 2024 16:29 |
| URI: | https://espace.inrs.ca/id/eprint/14779 |
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