Grover, Amit; Smith, Charles E.; Gregory, Mary; Cyr, Daniel G. 
ORCID: https://orcid.org/0000-0002-6566-783X; Sairam, M Ram et Hermo, Louis
(2005).
Effects of FSH receptor deletion on epididymal tubules and sperm morphology, numbers, and motility
Molecular Reproduction and Development
, vol. 72
, nº 2.
pp. 135-144.
DOI: 10.1002/mrd.20303.
Résumé
Follicle stimulating hormone (FSH) interacts with its cognate receptor (R) on Sertoli cells within the testis and plays an important role in the maintenance of spermatogenesis. Male FSH-R knockout (FORKO) mice show fewer Sertoli cells and many that are structurally abnormal and as a consequence fewer germ cells. Lower levels of serum testosterone (T) and androgen binding protein (ABP) also occur, along with reduced fertility. To assess the effects of FSH-R depletion as an outcome of testicular abnormalities, sperm from the cauda epididymidis were counted and examined ultrastructurally. As reduced fertility may also reflect changes to the epididymis, the secondary responses of the epididymis to lower T and ABP levels were also examined by comparing differences in sizes of epididymal tubules in various regions of FORKO and wild type (WT) mice. Sperm motility was evaluated in FORKO mice and compared to that of WT mice by computer assisted sperm analysis (CASA). Quantitatively, the data revealed that epithelial areas of the caput and corpus epididymidis were significantly smaller in FORKO mice compared to WT mice. Cauda epididymal sperm counts in FORKO mice were also much lower than in WT mice. This resulted in changes to 9 out of 14 sperm motility parameters, related mostly to velocity measures, which were significantly lower in the FORKO mice. The greatest change was observed relative to the percent static sperm, which was elevated by 20% in FORKO mice compared to controls. EM analyses revealed major changes to the structure of the heads and tails of cauda luminal sperm in FORKO mice. Taken together these data suggest a key role for the FSH receptor in maintaining Sertoli cells to sustain normal sperm numbers and proper shapes of their heads and tails. In addition, the shrinkage in epididymal epithelial areas observed in FORKO mice likely reflect direct and/or indirect changes in the functions of these cells and their role in promoting sperm motility, which is noticeably altered in FORKO mice.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | gene knockout; FORKO mouse; epididymis; sperm counts and motility |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 01 nov. 2024 19:46 |
| Dernière modification: | 01 nov. 2024 19:46 |
| URI: | https://espace.inrs.ca/id/eprint/14727 |
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