Goncalves, David M. et Girard, Denis 
ORCID: https://orcid.org/0000-0002-3342-5027
(2011).
Titanium dioxide (TiO2) nanoparticles induce neutrophil influx and local production of several pro-inflammatory mediators in vivo
International Immunopharmacology
, vol. 11
, nº 8.
pp. 1109-1115.
DOI: 10.1016/j.intimp.2011.03.007.
Résumé
We have recently described the in vitro effects of titanium dioxide (TiO2) nanoparticles (NPs) in human neutrophils. The objective of the present study was to det. if TiO2 NPs induced leukocyte infiltration in the in vivo murine air pouch model of acute inflammation and, if so, to identify which of the main cell populations were attracted. TiO2 NPs induced leukocyte influx after 3-9 h of treatment, since the no. of leukocytes attracted significantly increased when compared to controls. Neutrophils are the most abundant leukocytes attracted by TiO2 representing more than ∼ 80% of cells, with the rest being mononuclear cells. Using an antibody array technique to detect 40 different analytes, we obsd. that TiO2 increased the local prodn. of several analytes in the exudates, including diverse chemokines. Using an ELISA assay, we found that the concn. of TiO2-induced MIP-1β was significantly increased vs controls, corroborating the antibody array results.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Inflammation; In vivo; Neutrophils; Nanoparticles; Cytokines; Antibody array |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 25 mars 2024 15:02 |
| Dernière modification: | 25 mars 2024 15:02 |
| URI: | https://espace.inrs.ca/id/eprint/14715 |
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