Girard, Denis 
ORCID: https://orcid.org/0000-0002-3342-5027; Boiani, Norman et Beaulieu, André D.
(1998).
Human Neutrophils Express the Interleukin-15 Receptor Alpha Chain (Il-15r Alpha) but Not the Il-9r Alpha Component
Clinical Immunology and Immunopathology
, vol. 88
, nº 3.
pp. 232-240.
DOI: 10.1006/clin.1998.4576.
Résumé
The interleukin-15 receptor (IL-15R) is composed of at least three chains, namely gamma(c), IL-2R beta, and the recently identified IL-15R alpha, while the IL-9R complex consists of gamma(c) and a subunit designated IL-9R alpha. Our previous work and that of others have shown that human neutrophils express gamma(c) and IL-2R beta (two components shared with IL- 2R) but not IL-2R alpha and that IL-15 is a neutrophil agonist, whereas IL-2 is not. In this study, using flow cytometry with a specific anti-human IL-15R alpha, we show for the first time that human neutrophils express surface IL-15R alpha. Although we previously found that IL-15 is a neutrophil agonist, our present work shows that IL-15 does not trigger superoxide production nor cell spreading onto glass. In addition, we report that human neutrophils do not respond to IL-9 with respect to the functions/responses studied, namely, superoxide production spreading onto glass, cell shape changes, phagocytosis, RNA synthesis, and apoptosis. Further, our results show that neutrophils do not express IL-9R alpha as assessed by flow cytometry with a specific anti-human IL-9R alpha antibody that stains the transfected cell line BW-h9R used as positive control. Finally, our results indicate that gamma(c) expression was not modulated and remained stable for up to 24 h when neutrophils were stimulated with all currently known "gamma(c) users," namely, IL-2, IL-4, IL-7, IL-9, and IL- 15. We conclude that human neutrophils express all IL-IBR components on their surface, including IL-15R alpha, that IL-15 activates human neutrophils (as the IL-4 neutrophil agonist) by a mechanism which does not involve upregulation of gamma(c) cell surface expression, and that IL-9 is not a neutrophil agonist as demonstrated by the inability to modulate the tested functions/responses that correlate with lack of the IL-SR component, namely, IL-9R alpha. (C) 1998 Academic Press.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | interleukin-15 interleukin-9 receptors neutrophils |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 07 juill. 2025 18:32 |
| Dernière modification: | 07 juill. 2025 18:32 |
| URI: | https://espace.inrs.ca/id/eprint/14700 |
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