Giannopoulos, Paresa N.; Nassoury, Nasha; Lamontagne, Lucie; Guertin, Claude 
ORCID: https://orcid.org/0000-0002-0710-6970 et Rashidan, Kianoush Khajeh
(2005).
Choristoneura fumiferana granulovirus pk-1: A baculoviral protein kinase
Journal of Biochemistry and Molecular Biology
, vol. 38
, nº 4.
pp. 457-467.
DOI: 10.5483/bmbrep.2005.38.4.457.
Résumé
Open reading frame (ORF) 3 on the Choristoneura fumiferana granulovirus (ChfuGV), located in the 11 kb fragment of the BamHI genomic bank encodes a predicted 32-kDa putative kinase protein. Bioinformatics analysis on the predicted amino acid sequence of ChfuGV PK-1 revealed the existence of 11 catalytic subdomains. Sequence analysis within the 5'-untranslated region (5'-UTR) of ChfuGV pk-1 indicates the presence of both putative early and late promoter motifs, indicating that pk-1 may be expressed throughout the infection cycle. Promoter sequence analysis reveals that pk-1 is deprived of a TATA box and appears instead to be regulated by other cis-acting transcriptional regulatory elements. Temporal transcription analysis by RT-PCR confirms the appearance of transcripts detected from 2 h p.i. until 72 h p.i. Northern blot hybridization characterizes pk-1 transcription as a 1.2 kb transcript. Homology comparisons reveal that ChfuGV PK-1 protein is most closely related to Phthorimea operculalla granulovirus (PoGV) with 80% amino acid identity.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Apoptosis; Baculovirus Catalytic domain; Choristoneura fumiferana granulovirus; Kinase protein; Protein analysis; Transcription |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 01 nov. 2024 15:20 |
| Dernière modification: | 01 nov. 2024 15:20 |
| URI: | https://espace.inrs.ca/id/eprint/14696 |
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