Gabriel, Christelle; McMaster, W. Robert; Girard, Denis 
ORCID: https://orcid.org/0000-0002-3342-5027 et Descoteaux, Albert ORCID: https://orcid.org/0000-0002-0633-5309
(2010).
Leishmania donovani promastigotes evade the antimicrobial activity of neutrophil extracellular traps
Journal of Immunology
, vol. 185
, nº 7.
pp. 4319-4327.
DOI: 10.4049/jimmunol.1000893.
Résumé
Upon their recruitment to a site of infection and their subsequent activation, neutrophils release DNA and a subset of their granule content to form filamentous structures, known as neutrophil extracellular traps, which capture and kill microorganisms. In this study, we show that Leishmania promastigotes induced the rapid release of neutrophil extracellular traps from human neutrophils and were trapped by these structures. The use of Leishmania mutants defective in the biosynthesis of either lipophosphoglycan or GP63 revealed that these two major surface promastigote virulence determinants were not responsible for inducing the release of the surface protease neutrophil extracellular traps. We also demonstrate that this induction was independent of superoxide production by neutrophils. Finally, in contrast to wild-type Leishmania donovani promastigotes, mutants defective in lipophosphoglycan biosynthesis were highly susceptible to the antimicrobial activity of neutrophil extracellular traps. Altogether, our data suggest that neutrophil extracellular traps may contribute to the containment of L. donovani promastigotes at the site of inoculation, thereby facilitating their uptake by mononuclear phagocytes.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 30 juin 2024 18:00 |
| Dernière modification: | 30 juin 2024 18:00 |
| URI: | https://espace.inrs.ca/id/eprint/14678 |
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