Fournout, Sylvie; Dozois, Charles M. 
ORCID: https://orcid.org/0000-0003-4832-3936; Yerle, Martine; Pinton, Philippe; Fairbrother, John M.; Oswald, Eric et Oswald, Isabelle P.
(2000).
Cloning, chromosomal location, and tissue expression of the gene for pig interleukin-18
Immunogenetics
, vol. 51
, nº 4-5.
pp. 358-365.
DOI: 10.1007/s002510050630.
Résumé
Interleukin 18 (IL-18), a recently described cytokine, plays an important role in the cell-mediated immune response, in particular through its ability to induce the production of interferon (IFN)-gamma. We cloned pig IL-18 cDNA from the intestinal epithelial cell line IPI-2I using a reverse transcriptase-polymerase chain reaction method with primers derived from the human IL-18 sequence. The amino acid sequence deduced from pig IL-18 cDNA encodes a 192 amino-acid polypeptide that exhibits 92, 90, 81, and 71% similarity to IL-18 from horse, dog, human, and rodents (mouse and rat), respectively. Structural comparison of the IL-18 protein with IL-1alpha and IL-1beta showed that IL-18 shares several characteristics with the IL-1 cytokine family: the IL-1 signature-like sequence, a potential caspase-1 (ICE) cleavage site, and the presence of 12 predicted beta strands. Fluorescence in situ hybridization was used to localize the IL-18 gene on the short arm (p13) of pig chromosome 9. Analysis of IL-18 expression in different organs of piglets demonstrated that IL-18 mRNA is weakly expressed in the kidney and the lung. By contrast, we observed highly constitutive expression of IL-18 mRNA in the spleen, mesenteric lymph nodes, and the intestine, particularly in the small intestine, indicating a potential role for IL-18 as a first line of host defense in the intestinal mucosa.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Pig; Cytokine; Interleukin-18; Chromosomal location; Tissue expression |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 11 avr. 2025 12:50 |
| Dernière modification: | 11 avr. 2025 12:50 |
| URI: | https://espace.inrs.ca/id/eprint/14672 |
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